Surface proteome biotinylation combined with bioinformatic tools as a strategy for predicting pathogen interacting proteins (CROSBI ID 60533)
Prilog u knjizi | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Horvatić, Anita ; Kuleš, Josipa ; Guillemin, Nicolas ; Martinković, Franjo ; Štimac, Iva ; Mrljak, Vladimir ; Bhide, Mangesh
hrvatski
Surface proteome biotinylation combined with bioinformatic tools as a strategy for predicting pathogen interacting proteins
Constant advancements in methodology and mass spectrometry instrumentation, genome sequencing and bioinformatic tools have enabled the identification of numerous pathogen proteomes. Identifying the pathogen interacting proteins by means of high-throughput techniques is key for understanding pathogen invasion and survival mechanisms and in such a way proposing specific proteins as pharmaceutical targets. Herein we describe the methodology for the enrichment and identification of pathogen surface proteome using cell surface protein biotinylation followed by LC-MS/MS and bioinformatic analyses of such data. This strategy is to be employed for the determination of protein subcellular localization and prediction of potential pathogen interacting proteins.
Bioinformatics ; Biotinylation ; CELLO ; DAVID ; Interacting proteins ; LC-MS ; Subcellular localization ; Surface proteome
nije evidentirano
engleski
Surface proteome biotinylation combined with bioinformatic tools as a strategy for predicting pathogen interacting proteins
nije evidentirano
Bioinformatics ; Biotinylation ; CELLO ; DAVID ; Interacting proteins ; LC-MS ; Subcellular localization ; Surface proteome
nije evidentirano
Podaci o prilogu
83-96.
objavljeno
10.1007/978-1-4939-7604-1_9
Podaci o knjizi
Methods in Molecular Biology: Host-pathogen interactions, Methods and protocols
Medina, Carlos ; López-Baena, Francisco Javier
New York (NY): Springer ; Humana Press
2018.
978-1-4939-7603-4
1064-3745
1940-6029