engleski

Effects of UVB radiation and norflurazon on green and brown hydra

The aim of this study was to determine whether exposure to UVB radiation and herbicide norfluorazon had a mutagenic effect on freshwater invertebrate hydra. The experiment was performed in 60 ml volume glass dishes filled with 50 ml of aquarium water for control group and in 60 ml volume glass dishes filled with 50 ml of norfluorazon in concentrations of 2x10-6 or 2x10-7 mol/L. Specimens of green and brown hydra were parallely exposed to UVB radiation wavelength 254 nm. The second part of the experiment was performed in the same way except there was no radiation. In brown hydra, the most visible was the damage to the tentacles, while in green hydra were observed tentacle-like growths on the body of hydra. In some individuals of the experimental animals, there was no visible damage to buds while in others the buds had the form of a damaged polyp. Budding was observed in both species, while sexual reproduction was noticed mainly in samples of green hydra treated with herbicide as well in the control samples. Mortality was low in green hydra samples and radiation proved to be the cause of greater damage than exposure to herbicide. Histology slides of brown hydra showed surface cellular/tissue formations that require further investigation.

Hydra ; UVB radiation ; norfluorazon ; budding ; sexual reproduction

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