engleski

The correlation between plasma cell morphology and chromosomal abnormalities in myeloma cells detected with fluorescent in situ hybridisation (FISH)

Objective: To investigate the possible connection between morphological features of plasma cells(PC) and some of the most frequent chromosomal abnormalities in multiple myeloma (MM). Method: Bone marrow aspirate smears taken at the moment of diagnosis were analyzed for: del(13q14), del(13q34), del(17p), t(4 ; 14) and t(14 ; 16). Smears were stained according to short May- Gruenwald-Giemsa(MGG) protocol, areas with abundance of myeloma cells marked, slides destained, and the FISH analysis performed on selected areas. On MGG-stained slides, the frequency of morphological features and morphometrical data were assessed - chromatin, nucleoli, irregular nuclei, multinuclearity, paranuclear halo, “flaming” PCs, ragged cytoplasm, cytoplasmatic budding, Russell bodies, longest nuclear (ND) and cytoplasmatic diameter (CD) and the ND/CD ratio. The correlations were analyzed using t- test. Results: FISH was successful on 120/169 smears. The incidence of chromosomal abnormalities was as expected from the literature, only del(17p) showed higher frequency. Del(13q14) was correlated with a higher proportion of PCs with ragged cytoplasm. Del(13q14) and del(13q34) were connected with more multinucleated PCs. Cases with del(13q14) had lower ND/CD ratio. Patients with t(4 ; 14) had more frequent Russell bodies. Conclusion: Some morphological features of PCs in MM, seen in BM aspirates, could provide a hint towards the presence of certain chromosomal abnormalities with prognostic significance.

plasma cell morphology ; multiple myeloma ; chromosomal abnormalities

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