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Pregled bibliografske jedinice broj: 916733

The correlation between plasma cell morphology and chromosomal abnormalities in myeloma cells detected with fluorescent in situ hybridisation (FISH)


Seili-Bekafigo, Irena; Valković, Toni; Rajković Molek, Koraljka; Jonjić, Nives
The correlation between plasma cell morphology and chromosomal abnormalities in myeloma cells detected with fluorescent in situ hybridisation (FISH) // VIRCHOWS ARCHIV / Massi, Daniela (ur.).
Heidelberg, Germany: Springer, 2017. str. S142-S142 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
The correlation between plasma cell morphology and chromosomal abnormalities in myeloma cells detected with fluorescent in situ hybridisation (FISH)

Autori
Seili-Bekafigo, Irena ; Valković, Toni ; Rajković Molek, Koraljka ; Jonjić, Nives

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
VIRCHOWS ARCHIV / Massi, Daniela - Heidelberg, Germany : Springer, 2017, S142-S142

Skup
29th European Congress of Pathology

Mjesto i datum
Amsterdam, Nizozemska, 2 - 6.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Plasma cell morphology ; multiple myeloma ; chromosomal abnormalities

Sažetak
Objective: To investigate the possible connection between morphological features of plasma cells(PC) and some of the most frequent chromosomal abnormalities in multiple myeloma (MM). Method: Bone marrow aspirate smears taken at the moment of diagnosis were analyzed for: del(13q14), del(13q34), del(17p), t(4 ; 14) and t(14 ; 16). Smears were stained according to short May- Gruenwald-Giemsa(MGG) protocol, areas with abundance of myeloma cells marked, slides destained, and the FISH analysis performed on selected areas. On MGG-stained slides, the frequency of morphological features and morphometrical data were assessed - chromatin, nucleoli, irregular nuclei, multinuclearity, paranuclear halo, “flaming” PCs, ragged cytoplasm, cytoplasmatic budding, Russell bodies, longest nuclear (ND) and cytoplasmatic diameter (CD) and the ND/CD ratio. The correlations were analyzed using t-test. Results: FISH was successful on 120/169 smears. The incidence of chromosomal abnormalities was as expected from the literature, only del(17p) showed higher frequency. Del(13q14) was correlated with a higher proportion of PCs with ragged cytoplasm. Del(13q14) and del(13q34) were connected with more multinucleated PCs. Cases with del(13q14) had lower ND/CD ratio. Patients with t(4 ; 14) had more frequent Russell bodies. Conclusion: Some morphological features of PCs in MM, seen in BM aspirates, could provide a hint towards the presence of certain chromosomal abnormalities with prognostic significance.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE