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Pregled bibliografske jedinice broj: 916162

Measuring innate immune response in epithelial cells after adenovirus infection


Nestić, Davor; Kovačević, Alen; Van den Bosch, Jolien; Knežević, Jelena; Custers, Jerome; Ambriović Ristov, Andreja; Majhen, Dragomira
Measuring innate immune response in epithelial cells after adenovirus infection // 7. kongres Slovenskega mikrobiološkega društva : knjiga povzetkov / Vodovnik, Maša ; Kušar, Darja ; Marinšek Logar, Romana (ur.).
Bled: Slovensko mikrobiološko društvo, 2017. str. 158-158 (poster, podatak o recenziji nije dostupan, sažetak, znanstveni)


CROSBI ID: 916162 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Measuring innate immune response in epithelial cells after adenovirus infection

Autori
Nestić, Davor ; Kovačević, Alen ; Van den Bosch, Jolien ; Knežević, Jelena ; Custers, Jerome ; Ambriović Ristov, Andreja ; Majhen, Dragomira

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7. kongres Slovenskega mikrobiološkega društva : knjiga povzetkov / Vodovnik, Maša ; Kušar, Darja ; Marinšek Logar, Romana - Bled : Slovensko mikrobiološko društvo, 2017, 158-158

ISBN
978-961-90346-6-8

Skup
7. kongres Slovenskega mikrobiološkega društva (SMD 2017)

Mjesto i datum
Bled, Slovenija, 22.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
adenovirus ; innate immune response ; gene transfer and vaccination

Sažetak
Adenovirus vectors based on a serotype 5 (AdV5) are the most commonly investigated adenoviral vectors for gene therapy and vaccination. Limitations for using AdV5 as a vector are activation of the host innate immune responses as well as preexisting immunity to AdV5 wild type. The aim of this study was to investigate the differences in the activation of innate immune response after infection with AdV5 and low seroprevalent serotypes of adenovirus: AdV35, and AdV26, which may be used to overcome the preexisting immunity to AdV5. After infecting SK-OV-3 and A549 cell lines with replication deficient AdV5, AdV26 and AdV35, we measured the levels of mRNA and corresponding proteins for molecules known to be involved in innate immune response using qPCR, western blot and ELISA. We showed that in contrast to AdV5, infection with AdV26 and AdV35 increased expression of genes known to be involved in initiation of inflammation, namely IL-6, IL-8, IFN-α1, TNF-α and IL-1β. We also observed differences in adaptor molecules situated downstream of pattern recognition receptors and those involved in inflammasome formation. We hypothesize that there are differences in role of innate immune response in AdV26 and AdV35 infection which might be used for construction of vectors with better performances for DNA transfer and vaccination purposes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
UIP-2014-09-3912 - Razumijevanje puta ulaska Adenovirusa tipa 26 u stanicu: način poboljšanja vektora za vakcinaciju (AdVEntPathVac) (Majhen, Dragomira, HRZZ - 2014-09) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

www.smd.si

Citiraj ovu publikaciju:

Nestić, Davor; Kovačević, Alen; Van den Bosch, Jolien; Knežević, Jelena; Custers, Jerome; Ambriović Ristov, Andreja; Majhen, Dragomira
Measuring innate immune response in epithelial cells after adenovirus infection // 7. kongres Slovenskega mikrobiološkega društva : knjiga povzetkov / Vodovnik, Maša ; Kušar, Darja ; Marinšek Logar, Romana (ur.).
Bled: Slovensko mikrobiološko društvo, 2017. str. 158-158 (poster, podatak o recenziji nije dostupan, sažetak, znanstveni)
Nestić, D., Kovačević, A., Van den Bosch, J., Knežević, J., Custers, J., Ambriović Ristov, A. & Majhen, D. (2017) Measuring innate immune response in epithelial cells after adenovirus infection. U: Vodovnik, M., Kušar, D. & Marinšek Logar, R. (ur.)7. kongres Slovenskega mikrobiološkega društva : knjiga povzetkov.
@article{article, author = {Nesti\'{c}, Davor and Kova\v{c}evi\'{c}, Alen and Van den Bosch, Jolien and Kne\v{z}evi\'{c}, Jelena and Custers, Jerome and Ambriovi\'{c} Ristov, Andreja and Majhen, Dragomira}, year = {2017}, pages = {158-158}, keywords = {adenovirus, innate immune response, gene transfer and vaccination}, isbn = {978-961-90346-6-8}, title = {Measuring innate immune response in epithelial cells after adenovirus infection}, keyword = {adenovirus, innate immune response, gene transfer and vaccination}, publisher = {Slovensko mikrobiolo\v{s}ko dru\v{s}tvo}, publisherplace = {Bled, Slovenija} }
@article{article, author = {Nesti\'{c}, Davor and Kova\v{c}evi\'{c}, Alen and Van den Bosch, Jolien and Kne\v{z}evi\'{c}, Jelena and Custers, Jerome and Ambriovi\'{c} Ristov, Andreja and Majhen, Dragomira}, year = {2017}, pages = {158-158}, keywords = {adenovirus, innate immune response, gene transfer and vaccination}, isbn = {978-961-90346-6-8}, title = {Measuring innate immune response in epithelial cells after adenovirus infection}, keyword = {adenovirus, innate immune response, gene transfer and vaccination}, publisher = {Slovensko mikrobiolo\v{s}ko dru\v{s}tvo}, publisherplace = {Bled, Slovenija} }




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