Measuring innate immune response in epithelial cells after adenovirus infection (CROSBI ID 656997)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Nestić, Davor ; Kovačević, Alen ; Van den Bosch, Jolien ; Knežević, Jelena ; Custers, Jerome ; Ambriović Ristov, Andreja ; Majhen, Dragomira
engleski
Measuring innate immune response in epithelial cells after adenovirus infection
Adenovirus vectors based on a serotype 5 (AdV5) are the most commonly investigated adenoviral vectors for gene therapy and vaccination. Limitations for using AdV5 as a vector are activation of the host innate immune responses as well as preexisting immunity to AdV5 wild type. The aim of this study was to investigate the differences in the activation of innate immune response after infection with AdV5 and low seroprevalent serotypes of adenovirus: AdV35, and AdV26, which may be used to overcome the preexisting immunity to AdV5. After infecting SK-OV-3 and A549 cell lines with replication deficient AdV5, AdV26 and AdV35, we measured the levels of mRNA and corresponding proteins for molecules known to be involved in innate immune response using qPCR, western blot and ELISA. We showed that in contrast to AdV5, infection with AdV26 and AdV35 increased expression of genes known to be involved in initiation of inflammation, namely IL-6, IL-8, IFN-α1, TNF-α and IL-1β. We also observed differences in adaptor molecules situated downstream of pattern recognition receptors and those involved in inflammasome formation. We hypothesize that there are differences in role of innate immune response in AdV26 and AdV35 infection which might be used for construction of vectors with better performances for DNA transfer and vaccination purposes.
adenovirus ; innate immune response ; gene transfer and vaccination
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Podaci o prilogu
158-158.
2017.
objavljeno
Podaci o matičnoj publikaciji
Vodovnik, Maša ; Kušar, Darja ; Marinšek Logar, Romana
Bled: Slovensko mikrobiološko društvo
978-961-90346-6-8
Podaci o skupu
7. kongres Slovenskega mikrobiološkega društva (SMD 2017)
poster
22.09.2017-22.09.2017
Bled, Slovenija