Rala - Rapid layout module for de novo genome assembly (CROSBI ID 656819)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Vaser, Robert ; Šikić, Mile
engleski
Rala - Rapid layout module for de novo genome assembly
Rala is a standalone layout module intended for assembly of raw reads generated by third generation sequencing platforms. It consists of two parts, read preprocessing inspired by HINGE (Kamath et al, 2017), and assembly graph simplification as described in Miniasm (Li, 2016). In preprocessing, coverage graphs are generated from pairwise mappings using Minimap (Li, 2016) and are used to detect chimeric reads as well as reads from repetitive regions. Afterwards, the assembly graph is simplified with transitive reduction, trimming, bubble popping and a heuristic which untangles leftover junctions in the graph. As a side result, we show that the percentage of chimeric reads produced by either the Pacific Biosciences or Oxford Nanopore Technologies platforms is correlated with the read length.
de novo assembly, layout phase
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Podaci o prilogu
1-1.
2017.
objavljeno
Podaci o matičnoj publikaciji
ISMB/ECCB 2017
Podaci o skupu
ISMB/ECCB 2017
poster
21.07.2017-25.07.2017
Prag, Češka Republika