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Pregled bibliografske jedinice broj: 914434

Preparative two-step purification of recombinant H1.0 linker histone and its domains


Ivić, Nives; Bilokapić, Silvija; Halić, Mario
Preparative two-step purification of recombinant H1.0 linker histone and its domains // PLoS One, 12 (2017), 12; e0189040-15 doi:10.1371/journal.pone.0189040. (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 914434 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Preparative two-step purification of recombinant H1.0 linker histone and its domains

Autori
Ivić, Nives ; Bilokapić, Silvija ; Halić, Mario

Izvornik
PLoS One (1932-6203) 12 (2017), 12; E0189040-15

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
linker histone, purification, nucleosome, chromatin

Sažetak
H1 linker histones are small basic proteins that have a key role in the formation and maintenance of higher-order chromatin structures. Additionally, many examples have shown that linker histones play an important role in gene regulation, modulated by their various subtypes and posttranslational modifications. Obtaining high amounts of very pure linker histones, especially for efficient antibody production, remains a demanding and challenging procedure. Here we present an easy and fast method to purify human linker histone H1.0 overexpressed in Escherichia coli, as well as its domains: N-terminal/globular domain and C-terminal intrinsically disordered domain. This purification protocol relies on a simple affinity chromatography step followed by cation exchange due to the highly basic properties of histone proteins. Therefore, this protocol can also be applied to other linker histones. Highly pure proteins in amounts sufficient for most biochemical experiments can be obtained. The functional quality of purified H1.0 histone and its domains has been confirmed by pull-down, gel-mobility shift assays and the nuclear import assay.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Silvija Bilokapić (autor)

Avatar Url Nives Ivić (autor)

Poveznice na cjeloviti tekst rada:

doi fulir.irb.hr journals.plos.org journals.plos.org

Citiraj ovu publikaciju:

Ivić, Nives; Bilokapić, Silvija; Halić, Mario
Preparative two-step purification of recombinant H1.0 linker histone and its domains // PLoS One, 12 (2017), 12; e0189040-15 doi:10.1371/journal.pone.0189040. (međunarodna recenzija, članak, znanstveni)
Ivić, N., Bilokapić, S. & Halić, M. (2017) Preparative two-step purification of recombinant H1.0 linker histone and its domains. PLoS One, 12 (12), e0189040-15 doi:10.1371/journal.pone.0189040..
@article{article, year = {2017}, pages = {e0189040-15}, DOI = {10.1371/journal.pone.0189040.}, keywords = {linker histone, purification, nucleosome, chromatin}, journal = {PLoS One}, doi = {10.1371/journal.pone.0189040.}, volume = {12}, number = {12}, issn = {1932-6203}, title = {Preparative two-step purification of recombinant H1.0 linker histone and its domains}, keyword = {linker histone, purification, nucleosome, chromatin} }
@article{article, year = {2017}, pages = {e0189040-15}, DOI = {10.1371/journal.pone.0189040.}, keywords = {linker histone, purification, nucleosome, chromatin}, journal = {PLoS One}, doi = {10.1371/journal.pone.0189040.}, volume = {12}, number = {12}, issn = {1932-6203}, title = {Preparative two-step purification of recombinant H1.0 linker histone and its domains}, keyword = {linker histone, purification, nucleosome, chromatin} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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