engleski

Extended spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from dogs

Infections caused by Gram-negative multidrug- resistant bacterial pathogens have been reported with increasing frequency. Special concern is given to carbapenem-resistant and extended-spectrum beta-lactamase (ESBL)- producing Enterobacteriaceae that are, according to World Health Organization (WHO), on Global priority list of antibiotic-resistant bacteria that pose the greatest threat to human health. Moreover, WHO highlights the need for coordination between human and animal surveillance systems to improve the ‘one health’ approach to limit the spread of resistant bacteria. The aim of this study was to determine susceptibility to cephalosporins of Klebsiella pneumonia isolates originated from dogs. The antimicrobial susceptibility was determined by disk diffusion testing according to CLSI guidelines and interpreted according to CLSI breakpoints. Double-disk synergy test (DDST) was performed as confirmatory test for suspected ESBL-production. Screening testing revealed six strains of K. pneumoniae resistant to third generation of cephalosporins. The isolates were identified according to the procedure described by MARKEY et al. (2013) and identification was confirmed with commercial strip for biochemical identification API 20E (bioMerieux, France). DDST testing was positive for all six isolates and the presence of genes encoding broad and extended-spectrum β-lactamases was examined by PCR. In all isolates PCR testing revealed the presence of blaTEM and blaCTX_M genes. To our knowledge, this is the first report of ESBL-producing strains of K. pneumoniae isolated from animals in Croatia. It is important to monitor the prevalence of such strains and their possible spreading because they pose a great danger to human health.

Klebsiella pneumoniae, ESBL, dog, Croatia

nije evidentirano