engleski

Oxidative and antioxidative status of nuchal muscles in rats exposed to chronic and acute stress

Acute and chronic stress can induce the production of reactive oxygen species which exert a multitude of biological effects that ranges from physiological regulatory functions to damaging alterations and thus participating in the pathogenesis of a number of diseases. In order to estimate the impact of acute and chronic stress on oxidative and antioxidative response in nuchal muscles (Semispinalis capitis, Splenius capitis, Splenius cervicis) of male (M), female (NON-OV) and ovariectomized female (OV), Sprague-Dawley rats were exposed to acute and chronic stress protocol. As an indicator of oxidative damage, lipid peroxidation levels expressed in terms of thiobarbituric acid reactive substances (TBARS) were determined, while antioxidative status was determined by catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione S- transferase (GST) activities and oxidized/reduced glutathione (GSH/GSSG) ratio. Both acute and chronic stress significantly increased TBARS content in in nuchal muscles of all groups relative to control groups. Chronic stress decreased antioxidative defence mechanism in nuchal muscles of males and females. Acute stress did not have significant impact on the most of the antioxidative enzyme activities (CAT, GPX, GST, SOD and GR) in males and females, although GSH/GSSG ratio was reduced. Ovariectomy increased LPO and reduced some of antioxidative enzyme activities (CAT, GPX). Oxidative and antioxidative response to acute and chronic stress, in nuchal muscles of males and females, showed similarity in most of the measured parameters, and it differed from the response in ovariectomized females. Unlike acute stress, chronic stress disrupts antioxidative defence significantly more, thus it can cause changes in muscle contraction and muscle fatigue, which may lead to chronic pain in neck and headache.

acute stress ; chronic stress ; nuchal muscles ; oxidative stress ; ovariectomy

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