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izvor podataka: crosbi

Fibrin gel as a scaffold for skin substitute – production and clinical experience (CROSBI ID 245759)

Prilog u časopisu | pregledni rad (znanstveni) | domaća recenzija

Kljenak, Antun ; Tominac Trcin, Mirna ; Bujić, Marina ; Dolenec, Tamara ; Jevak, Martina ; Mršić, Gordan, Zmiš, Gordana ; Barčot, Zoran ; Muljačić, Ante ; Popović, Maja Fibrin gel as a scaffold for skin substitute – production and clinical experience // Acta clinica Croatica, 55 (2016), 2; 279-288. doi: 10.20471/acc.2016.55.02.15

Podaci o odgovornosti

Kljenak, Antun ; Tominac Trcin, Mirna ; Bujić, Marina ; Dolenec, Tamara ; Jevak, Martina ; Mršić, Gordan, Zmiš, Gordana ; Barčot, Zoran ; Muljačić, Ante ; Popović, Maja

engleski

Fibrin gel as a scaffold for skin substitute – production and clinical experience

The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaffolds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in fibrin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fluorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed. After hydrosurgical preparation of deep burn necrotic tissue, wound bed was prepared for skin substitutes. Progress of healing was documented using visual estimation and photos. Scanning electron microscope images showed good cell attachment and colony spreading of keratinocytes and fibroblasts on fibrin scaffold. Immunofluorescent staining of cell cultures on fibrin scaffold showed expression of vimentin, a marker of fibroblast cells, cytokeratin 19, a marker of epithelial stem cells, as well as involucrin, a marker of differentiated keratinocytes. Clinical results clearly showed that appearance of the skin did not differ significantly from the areas of transplanted skin using split-thickness skin graft techniques. In conclusion, using these fibrin- cultured autografts on massive full-thickness burn resulted in good healing.

Cells, cultured ; Fibrin ; Skin, artificial ; Burns

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Podaci o izdanju

55 (2)

2016.

279-288

objavljeno

0353-9466

1333-9451

10.20471/acc.2016.55.02.15

Povezanost rada

nije evidentirano

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