engleski

Identification of motor proteins in the bridging fiber of the mitotic spindle

At the onset of mitosis, cell constructs a spindle, a dynamic structure responsible for precise segregation of replicated genome between daughter cells. This unique cytoskeletal apparatus is made of chromosomes, microtubules and microtubule-associated proteins. Recent studies have begun to uncover a new class of microtubules, termed bridging microtubules, that span the region between sister kinetochores, whilst forming the bridging fiber that laterally connects sister k-fibers. In this thesis, I have identified localization of motor microtubule-associated proteins in the mitotic spindle with respect to the bridging fiber positions. MKLP1, a kinesin-6 motor, was observed to enrich the central part of the spindle at the positions of the bridging fibers. Overexpression of PRC1, an anti-parallel cross-linker, which is confirmed in the bridging fibers, shows correlation of MKLP1 localization with PRC1-labeled bridging overlaps. Additionally, silencing of Kif4A, a major binding partner of PRC1, elongates overlap regions of the bridging fibers.

motor protein, kinesin, dynein, PRC1, bridging microtubules, mitosis

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