engleski

Encapsulation of new biological active peptides into liposomes

In this work we examined the encapsulation of the new immunomodulators of peptidoglycan structure and synthetic adamantyltripeptides into the liposomes with the aim to possibly increase and optimize their therapeutic efficacy. Our studies concern two groups of immunomodulators: a) peptidoglycan monomer (PGM, b-D-GlcNAc-(1&reg ; 4)-MurNAc-L-Ala-D-isoGln-meso-A2pm(eNH2)-D-Ala-D-Ala) and its two semisynthetic derivatives, Boc-L-tyrosyl-peptidoglycan monomer and (adamant-1-yl)-acetyl peptidoglycan monomer ; b) synthetic adamantyltripeptides comprising a part of peptidoglycan structure (D- and L-(adamant-2-yl)-Gly-L-Ala-D-isoGln). All mentioned compounds were shown to exhibit versatile biological activity 1, 2, 3. The large multilamellar negatively charged vesicles using egg lecithin were prepared. The liposome preparations were chromatographed on the Sephadex G-50 or centrifuged in ultracentrifuge. The entrapment of tested compounds and the stability of respective preparations were followed in two ways: by the use of 14C-labelled peptidoglycans or by HPLC. The influence of chemical structure on the amount of entrapped material into liposomes will be shown. 1. J. Tomašić, and I.Hršak, , Peptidoglycan monomer originating from Brevibacterium divaricatum - its metabolism and biological activities in the host. In: Surface Structures of Microorganisms and Their Interactions with the Mammalian Host (Schrinner, E. et al., Ed.) VCH, Weinheim, 1987, str. 113-121 2. B. Vranešić, J. Tomašić, S. Smerdel, D. Kantoci and F. Benedetti, Helv. Chim. Acta 76 (1993), 1752-1758. 3. J. Tomašić, I. Hanzl-Dujmović, B. Špoljar, B. Vranešić, M. Šantak, A. Jovičić, Vaccine 8 (2000) 1236-1243.

nije evidentirano

nije evidentirano