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Pregled bibliografske jedinice broj: 911882

Hyperbaric oxygenation and 20- hydroxyeicosatetreanoic acid inhibition reduce stroke volume in female diabetic Sprague–Dawley rats


Mišir, Mihael; Renić, Marija; Novak, Sanja; Mihalj, Martina; Ćosić, Anita; Vesel, Monika; Drenjančević, Ines
Hyperbaric oxygenation and 20- hydroxyeicosatetreanoic acid inhibition reduce stroke volume in female diabetic Sprague–Dawley rats // Experimental physiology, 102 (2017), 12; 1596-1606 doi:10.1113/EP086402 (međunarodna recenzija, članak, znanstveni)


Naslov
Hyperbaric oxygenation and 20- hydroxyeicosatetreanoic acid inhibition reduce stroke volume in female diabetic Sprague–Dawley rats

Autori
Mišir, Mihael ; Renić, Marija ; Novak, Sanja ; Mihalj, Martina ; Ćosić, Anita ; Vesel, Monika ; Drenjančević, Ines

Izvornik
Experimental physiology (0958-0670) 102 (2017), 12; 1596-1606

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Brain ischemia ; stroke ; hyperbaric oxygenation ; diabetes mellitus

Sažetak
We evaluated the effects of acute and repetitive hyperbaric oxygenation (HBO2), 20- HETE inhibition by N-hydroxy-N'-(4-butyl- 2methylphenyl)-formamidine (HET0016) and their combinations on experimental stroke outcomes. Streptozotocin-induced type-1 diabetic Sprague- Dawley female rats (N=42, N=7/group), were subjected to 30’ transient middle cerebral artery occlusion (t-MCAO) / reperfusion and divided in following groups ; a) Control group, without treatment ; and groups exposed to: b) HBO2 ; c) multiple HBO2 (HBO2 immediately and second exposure 12h after t-MCAO) ; d) HET0016 pretreatment (1mg/kg/3 days prior to t-MCAO) combined with HBO2 after t-MCAO ; e) HET0016 treatment (1h before, during and for 6 hours after t-MCAO) ; and f) HET0016 treatment followed by HBO2 after t-MCAO. mRNA expression of CYP2J3, CYP2C11, CYP4A1, eNOS and EPHX2 was determined by real-time qPCR. Cortical infarct size and total infarct size were equally significantly reduced in HBO2 and HET0016 treated rats. Combined treatment with HET0016 and HBO2 provided no significant additive effect compared to HET0016 treatment only. mRNA of Cyp2J3 was significantly increased in all study groups, and mRNA of Cyp2C11 was significantly increased in multiple HBO2 and HET0016 treatment followed by HBO2 groups, compared to Control. eNOS expression was significantly increased after HBO2 treatments and expression of EPHX2 was increased in all groups compared to Control group. In diabetic female SD rats HBO2 and HET0016 are highly effective stroke treatments, suggesting involvement of CYP450 metabolites and NO pathway in this therapeutic effect.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
219-2160133-2034 - Djelovanje kisika na vaskularnu funkciju u zdravlju i bolesti (Ines Drenjančević, )
VIF-2016 MEFOS (PI Drenjancevic Ines)

Ustanove
Medicinski fakultet, Zagreb,
Medicinski fakultet, Osijek

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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