engleski

Genetic relatedness among basil (Ocimum spp.) accessions using RAPD markers

Genetic relatedness among basil accessions including six species of Ocimum (O. basilicum L., O. americanum L., O. x citriodorum L., O. minimum L., O. gratissiumum L., O. tenuiflorum L.) and six botanical varieties or cultivars of O. basilicum L. [var. basilicum L. cv. Genovese, var. basilicum L. cv. Sweet Basil, var. difforme Benth., var. purpurascens Benth., cv. Dark Opal, and var. thyrsiflorum (L.) Benth.] were analyzed by random amplified polymorphic DNA (RAPD) markers. Five primers were used for amplification. They yielded a total of 102 easily scorable polymorphic markers. Jaccard indices were calculated and phylogenetic relationships were determined by neighbor-joining cluster analysis. Different Ocimum species were clearly divided into separate clusters with the exception of O. minimum accessions, which clustered together with O. basilicum accessions. In addition to morphological, chemical and crossability data, RAPD analysis can be a useful tool for resolving existing problems in identification and classification of basils.

basil; Ocimum; germplasm characterization; molecular taxonomy; molecular marker

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