engleski

Serological evidence of bluetongue and a preliminary entomological study in southern Croatia

In December 2001, bluetongue (BT) was confirmed serologically by the Croatian Veterinary Institute using the competitive enzyme-linked immunosorbent assay (c-ELISA). Results of the serological testing of blood samples from ruminants in the Dubrovacko-Neretvanska County are presented (3 318 sera of ruminants from 53 herds were examined). In total, 357 bovine sera (178 or 49.9% positive), 1 268 ovine sera (174 or 13.7% positive) and 1 693 caprine sera (270 or 15.9% postive) were tested. Antibodies to BT virus serotype 9 were detected in 212 of the positive sera by serum neutralisation. A preliminary light-trap survey for midges of the Culicoides genus was also performed in the Dubrovacko-Neretvanska County. Fourteen light-trap collections from seven locations were examined and yielded a total of 4 872 Culicoides of which 4 492 (92%) belonged to the Obsoletus Complex (including C. obsoletus and C. scoticus).

Bluetongue, Competitive enzyme-linked immunosorbent assay, Croatia, Culicoides, Light trap, Ruminant, Serum neutralisation test

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