engleski

“Tumor Bank“ of bone marrow and peripheral blood malignant cells among patients with acute types of leukemia and chronic lymphocytic leukemia

Bone marrow and peripheral blood are ideal samples for cell suspension and sample preservation in liquid nitrogen at -196˚C, in which cells remain viable and are thereby sufficient for subsequent analysis methods. Aim of the study: To analyse the degree of success in obtaining suspension in patients with acute leukaemia (AL) and chronic lymphocytic leukaemia (CLL) in relation to number of leucocytes in peripheral blood. Material and Methods: A total of 98 bone marrow and peripheral blood samples were analysed – 40 samples were taken from patients suffering from AL and 58 from patients suffering from KLL. Samples containing 5 ml of bone marrow with 1 ml of heparin and 10 ml of peripheral blood with anticoagulant were diluted in a physiological solution and then washed in a physiological solution after the mononuclears was separated with ficoll. Cells re-suspended in an RPMI medium, which functions as a form of cell preservation, were divided into aliquots and placed in liquid nitrogen at -196˚C after mononuclear concentration had been established. Results: In 16 patients with ALL and 10 patients with CLL number of leucocytes in peripheral blood reached levels of up to 10x109/L, in 4 patients with AL and 16 patients with KLL levels were up to 10-20x109/L, in 4 patients with AL and 16 patients with CLL levels were up to 20-30x109/L and in 16 patients with AL and 16 patients with CLL levels were >30x109/L. In the first three groups with the highest number of leucocytes in peripheral blood the concentration of mononuclear cells after suspension was constant and varied from 1, 7-3, 96x109/L in the AL group, and from 2, 9-4, 87x109/L in the CLL group ; the number of individual samples was 3-8. A significantly higher concentration was obtained in both groups with bone marrow and peripheral blood suspension in cases where the leukocyte count was higher than 30x109/L: 12, 56x109/L with AL and 13, 03x109/L with CLL, whereby there was a maximum number of aliquots (10) in all cases. Conclusion: The concentration of mononuclear cells in suspension of bone marrow and peripheral blood among AL and CLL samples after separation with ficoll is higher if leukocyte numbers, as well as pathological cells numbers in the bone marrow are higher. However, it is possible to obtain a concentration of cells sufficient for individual samples (aliquots) even when there are smaller numbers of leukocytes.

Tumor ban ; bone marrow ; lymph node ; acute leukemia ; chronic lymphocytic leukemia

nije evidentirano