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Pregled bibliografske jedinice broj: 909499

HCE-T cell-based permeability model: A well- maintained or a highly variable barrier phenotype?


Juretić, Marina; Jurišić Dukovski, Bisera; Krtalić, Iva; Reichl, Stephan; Cetina-Čižmek, Biserka; Filipović-Grčić, Jelena; Lovrić, Jasmina; Pepić, Ivan
HCE-T cell-based permeability model: A well- maintained or a highly variable barrier phenotype? // European journal of pharmaceutical sciences, 104 (2017), 23-30 doi:10.1016/j.ejps.2017.03.018 (međunarodna recenzija, članak, znanstveni)


Naslov
HCE-T cell-based permeability model: A well- maintained or a highly variable barrier phenotype?

Autori
Juretić, Marina ; Jurišić Dukovski, Bisera ; Krtalić, Iva ; Reichl, Stephan ; Cetina-Čižmek, Biserka ; Filipović-Grčić, Jelena ; Lovrić, Jasmina ; Pepić, Ivan

Izvornik
European journal of pharmaceutical sciences (0928-0987) 104 (2017); 23-30

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cornea ; Barrier properties ; Drug permeability ; In vitro/ex vivo correlation ; Ocular delivery

Sažetak
The most extensively characterized human- derived cell line used in transcorneal permeability studies, in terms of passive transcellular and paracellular transport, transporter expression and metabolic enzymes, is the immortalized human corneal epithelial cell line (HCE-T). The purpose of this study is to describe the changes in the HCE-T barrier phenotype in vitro when valid cultivation conditions, in accordance with the standardized HCE-T cell-based model protocol, were employed. Evaluation of the structural and functional barrier properties revealed two different HCE-T barrier phenotypes, depending on the polycarbonate membrane pore size. Model I (pore size 0.4 μm) was characterized by a multilayered HCE-T epithelium at the apical side and a weak barrier function (70–115 Ω × cm2), whereas Model II (pore size 3 μm) consisted of an apical lipophilic HCE-T monolayer and a basolateral lipophilic monolayer of migrated HCE-T cells that showed improved barrier properties (1700–2600 Ω × cm2) compared with Model I. Considering the permeation of ophthalmic compounds and in vitro/ex vivo correlation, Model II was better able to predict transcorneal drug permeation. This study highlights the important aspects of HCE-T barrier phenotype variability that should be continuously monitored in the routine application of HCE-T cell-based models across both academic and pharmaceutical industry research laboratories.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija



POVEZANOST RADA


Projekt / tema
HRZZ-PA-04.01/56

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb

Citiraj ovu publikaciju

Juretić, Marina; Jurišić Dukovski, Bisera; Krtalić, Iva; Reichl, Stephan; Cetina-Čižmek, Biserka; Filipović-Grčić, Jelena; Lovrić, Jasmina; Pepić, Ivan
HCE-T cell-based permeability model: A well- maintained or a highly variable barrier phenotype? // European journal of pharmaceutical sciences, 104 (2017), 23-30 doi:10.1016/j.ejps.2017.03.018 (međunarodna recenzija, članak, znanstveni)
Juretić, M., Jurišić Dukovski, B., Krtalić, I., Reichl, S., Cetina-Čižmek, B., Filipović-Grčić, J., Lovrić, J. & Pepić, I. (2017) HCE-T cell-based permeability model: A well- maintained or a highly variable barrier phenotype?. European journal of pharmaceutical sciences, 104, 23-30 doi:10.1016/j.ejps.2017.03.018.
@article{article, year = {2017}, pages = {23-30}, DOI = {10.1016/j.ejps.2017.03.018}, keywords = {Cornea, Barrier properties, Drug permeability, In vitro/ex vivo correlation, Ocular delivery}, journal = {European journal of pharmaceutical sciences}, doi = {10.1016/j.ejps.2017.03.018}, volume = {104}, issn = {0928-0987}, title = {HCE-T cell-based permeability model: A well- maintained or a highly variable barrier phenotype?}, keyword = {Cornea, Barrier properties, Drug permeability, In vitro/ex vivo correlation, Ocular delivery} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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