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Effects of pentadecapeptide bpc 157, l-name and l-arginine on serial rib fracture in the rats

Introduction: Numerous studies have shown the beneficial effects of pentadecapeptide BPC 157 application in tissue healing. The hypothesis of this study was that the use of BPC 157 accelerates and improves healing of surgically induced serial rib fracture in rats. The objectives of this study were to analyze the effects of BPC 157 in healing of surgically induced injury in rats, on the level of clinical and functional outcomes and morphological changes in the examination of the macroscopic characteristics with the help of X- ray, computed tomography (CT) and biomehanical testing. Materials and methods: We used male Albino Wistar rats, 400 g body weight, kept under normal conditions, 5 rats at least per each experimental group and period, randomly assigned for all of the experiments, approved by our Local Ethics Committee. The method we used in causing serial rib fracture was the one described by Hashimoto et al. (1989). In anaesthetized rats, an incision was made on the right lateral aspect of the trunk between the eight and tenth rib. Previously mentioned ribs were located and fractured 2 cm from the vertebral column using a pair of fine scissors. Postoperatively the experimental animals were divided into thirteen groups according to the mode of substance application. The animals were sacrificed according to the study protocol on twentieth and on the sixtieth day after surgical rib fractures. After the sacrifice of the animals, macroscopic evaluation and further verification of the results by X-ray, CT and biomehanical testing was made. Results: In animals treated with BPC 157 bridging occurs earlier in fracture crack, no overproduction of callus and creation of pseudoarthroses was found in later course of the healing process.We also found that the pleural adhesions in control group were significant unlike the treated group were we found no adhesions. Discussion: The present macroscopic and radiological examinations results confirm our hypothesis but the biochemical (serum levels of calcium, phosphate and acid-base status) and histopathological analysis is still in progress. Acknowledgments: I would like to thank my mentor for making this experiment possible.

pentadecapeptide BPC 157 ; L-NAME ; L-arginine ; serial rib fracture ; rats

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