Cyclodextrin encapsulation of daidzein and genistein by grinding: Implication on the glycosaminoglycan accumulation in mucopolysaccharidosys type II and III fibroblasts (CROSBI ID 244633)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Fumić, Barbara ; Jablan, Jasna ; Cinčić, Dominik ; Zovko Končić, Marijana ; Jug, Mario
engleski
Cyclodextrin encapsulation of daidzein and genistein by grinding: Implication on the glycosaminoglycan accumulation in mucopolysaccharidosys type II and III fibroblasts
This work was aimed to investigate the potential effect of cyclodextrin encapsulation on intrinsic ability of daidzein (DAD) and genistein (GEN) to inhibit the glycosaminoglycan (GAG) synthesis in fibroblasts originating from patients with mucopolysaccharidoes (MPS), type II and III. DAD or GEN encapsulation with either 2-hydroxypropyl-β-cyclodextrin or sulphobuthylether-β-cyclodextrin were achieved by neat grinding and characterised by thermal analysis, X-ray powder diffraction, scanning electron microscopy and solubility testing, confirming the complexes formation with increased solubility with respect to starting compounds. Both isoflavones, as well as their co-ground cyclodextrin complexes reduced GAG levels in the fibroblasts of MPS II and MPS III patient for 54.8-77.5 %, in a dose dependant manner, without any significant cytotoxic effect. Cyclodextrin encapsulation did not change the intrinsically high effect of both DAD and GEN on the GAG level reduction in the treated cells, thus could be considered as a part of combination therapies of MPS.
daidzein ; genistein ; cyclodextrin ; neat grinding ; glycosaminoglycans
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Podaci o izdanju
35 (1)
2018.
1-12
objavljeno
0265-2048
1464-5246
10.1080/02652048.2017.1409819