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The estimation of oxime efficiency is affected by the experimental design of phosphylated acetylcholinesterase reactivation (CROSBI ID 244595)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Maček Hrvat, Nikolina ; Zorbaz, Tamara ; Šinko, Goran ; Kovarik, Zrinka The estimation of oxime efficiency is affected by the experimental design of phosphylated acetylcholinesterase reactivation // Toxicology letters, 293 (2018), 222-228. doi: 10.1016/j.toxlet.2017.11.022

Podaci o odgovornosti

Maček Hrvat, Nikolina ; Zorbaz, Tamara ; Šinko, Goran ; Kovarik, Zrinka

engleski

The estimation of oxime efficiency is affected by the experimental design of phosphylated acetylcholinesterase reactivation

Reactivation of acetylcholinesterase (AChE), an essential enzyme in neurotransmission, is a key point in the treatment of acute poisoning by nerve agents and pesticides, which structurally belong to organophosphorus compounds(OP). Due to the high diversity of substituents on the phosphorous atom, there is a variety of OP-AChE conjugates deriving from AChE inhibition, and therefore not only is there no universal reactivator efficient enough for the most toxic OPs, but for some nerve agents there is still a lack of any reactivator at all. The endeavor of many chemists to find more efficient reactivators resulted in thousands of newly-designed and synthesized oximes−potential reactivators of AChE. For an evaluation of the oxime's reactivation efficiency, many research groups employ a simple spectrophotometric Ellman method. Since parameters that describe reactivator efficiency are often incomparable among laboratories, we tried to emphasize the critical steps in the determination of reactivation parameters as well as in the experimental design of a reactivation assay. We highlighted the important points in evaluation of reactivation kinetic parameters with an aim to achieve better agreement and comparability between the results obtained by different laboratories and overall, a more efficient evaluation of in vitro reactivation potency.

Cholinesterase ; 2-PAM ; HI-6 ; Sarin ; Nerve agent ; Ellman assay

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Podaci o izdanju

293

2018.

222-228

objavljeno

0378-4274

1879-3169

10.1016/j.toxlet.2017.11.022

Povezanost rada

Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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