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Morfološka i proteomska analiza bičaša roda Trypanosoma na području Republike Hrvatske (CROSBI ID 655202)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Martinković, Franjo Morfološka i proteomska analiza bičaša roda Trypanosoma na području Republike Hrvatske // Book of Abstracts 7th International Congress "Veterinary Science and Profession". / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran (ur.). Zagreb, 2017. str. 162-162

Podaci o odgovornosti

Martinković, Franjo

hrvatski

Morfološka i proteomska analiza bičaša roda Trypanosoma na području Republike Hrvatske

The species of Trypanosoma genus are spread worldwide. Some species can cause serious health problems in humans and animals, although some species are apathogenic for their host. In Croatia apathogenic species were isolated: T. melophagium from sheep ked, T. theileri from cattle, Trypanosoma sp. from cervids and T. rotatorium from frogs. For seroepizootiological studies as antigen source, in vitro cultured trypanosomes are used. Except by in vitro cultivation, antigen research can be done with proteomic analysis. The aims of this study were to describe T. rotatorium bloodstream forms based on morphometric parameters, in vitro cultivation of isolated tripanosomes in new in vitro culture system medium as antigen source for further seroepizootiological research and their proteomic profiling. Also, by in vitro cultivation, goal is to describe the vitality and morphology of all the trypanosome species present in Croatia and culture the bloodstream forms for further seroepizootiological investigations. Proteomic analysis of trypanosome isolates will elucidate their differences and similarities. T. rotatorium morphology was described using standard morphometric parameters. A new in vitro culture, Biphasic Chocolate Agar for Trypanosomatids was compared with Liver Tryptose Medium by in vitro cultivation of isolated trypanosomes. Proteomic profile of trypanosomes was analyzed by two dimensional electrophoresis and mass spectrometry. Morphometric analysis of T. rotatorium bloodstream forms showed high polymorphism. By culturing the trypanosomes in new culture system, antigen for further seroepidemiological research was produced. Proteomic analysis showed marked difference between analyzed trypanosome flagellates. This is for the first time that T. rotatorium bloodstream forms were analyzed in Croatia. We showed here a new in vitro culture system media. For the first time proteome profile of T. rotatorium, T. theileri, T. melophagium and cervid trypanosome were presented.

morfologija, Trypanosoma, in vitro uzgoj, proteomska analiza

nije evidentirano

engleski

Morphological and proteomic analysis of flagellates from Trypanosoma genus in Croatia.

nije evidentirano

morphology, Trypanosoma, in vitro cultivation, proteomic analysis

nije evidentirano

Podaci o prilogu

162-162.

2017.

objavljeno

Podaci o matičnoj publikaciji

Book of Abstracts 7th International Congress "Veterinary Science and Profession".

Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran

Zagreb:

Podaci o skupu

7th International Congress Veterinary Science and Profession.

pozvano predavanje

05.10.2017-07.10.2017

Zagreb, Hrvatska

Povezanost rada

nije evidentirano