engleski

Genetic diversity of three donkey populations in the Croatian coastal region.

Genetic variation in three Croatian donkey populations, Istrian (IS), North Adriatic (NA) and Littoral-Dinaric (LD), was analysed using eight microsatellite loci and by sequencing and SSCP analysis of the proximal part of the mtDNA D-loop region. The analysis of microsatellite loci revealed observed heterozygosities in the range from 0.37 (MPZ002 in LD) to 0.85 (AHT21 in LD) and PIC values in the range from 0.36 (MPZ002 in NA) to 0.78 (AHT21 in LD). The overall probability of exclusion was 0.991. Two populations (IS and NA) were closely related (Fst = 0.0034), whereas genetic distances between IS and LD (Fst = 0.0212) and NA and LD (Fst = 0.0267) were higher. Partitioning of genetic diversity using AMOVA revealed 97.61% of the total genetic variance within populations, and 2.73% between Littoral-Dinaric population and Istrian/North Adriatic population group. Sequencing of the proximal part of the mtDNA D-loop region revealed 36 polymorphic sites representing 19 haplotypes which clustered into three haplotype groups (Y, W, Ws). The Y haplotype was found only in the IS population which is characterised by a large body size and haplotypes W and Ws were only found in the NA and LD population with smaller animals. However, all three haplotypes were found in LD population, indicating sporadic migration events from the IS into LD donkey population.

donkey; genetic diversity; microsatellite; D-loop; SSCP

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