Napredna pretraga

Pregled bibliografske jedinice broj: 904947

Copper-phospholipid interaction at cell membrane model hydrophobic surfaces


Mlakar, Marina; Cuculić, Vlado; Frka, Sanja; Gašparović, Blaženka
Copper-phospholipid interaction at cell membrane model hydrophobic surfaces // Bioelectrochemistry (Amsterdam), 120 (2018), C; 10-17 doi:10.1016/j.bioelechem.2017.11.004 (međunarodna recenzija, članak, znanstveni)


Naslov
Copper-phospholipid interaction at cell membrane model hydrophobic surfaces

Autori
Mlakar, Marina ; Cuculić, Vlado ; Frka, Sanja ; Gašparović, Blaženka

Izvornik
Bioelectrochemistry (Amsterdam) (1567-5394) 120 (2018), C; 10-17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Phospholipid membrane ; Copper (II) mixed ligand complex ; Voltammetry Langmuir monolayer ; Electrophoretic zeta potential

Sažetak
Detailed investigation of Cu (II) binding with natural lipid phosphatidylglycerol (PG) in aqueous solution was carried out by voltammetric measurements at the mercury drop electrode, complemented by monolayer studies in a Langmuir trough and electrophoretic measurements, all used as models for hydrophobic cell membranes. Penetration of copper ions into the PG layer was facilitated by the formation of hydrophilic Cu- Phenanthroline (Phen) complex in the subphase, followed by the mixed ligand Cu-Phen-PG complex formation at the hydrophobic interface. Electrophoretic measurements indicated a comparatively low abundance of the formed mixed ligand complex within the PG vesicles, resulting it the zeta potential change of + 0.83 mV, while monolayer studies confirmed their co-existence at the interface. The Cu- Phen-PG complex was identified in the pH range from 6 to 9. The stoichiometry of the complex ([PhenCuOHPG]), as well as its stability and kinetics of formation, were determined at the mercury drop electrode. Cu-Phen-PG reduces quasireversibly at about − 0.7 V vs. Ag/AgCl including reactant adsorption, followed by irreversible mixed complex dissociation, indicating a two-electron transfer - chemical reaction (EC mechanism). Consequently, the surface concentration (γ) of the adsorbed [PhenCuOHPG] complex at the hydrophobic electrode surface was calculated to be (3.35 ± 0.67) × 10− 11 mol cm− 2. Information on the mechanism of Cu (II) - lipid complex formation is a significant contribution to the understanding of complex processes at natural cell membranes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-8607 - Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu (Blaženka Gašparović, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati