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Construction of novel Saccharomyces cerevisiae hybrid strains resistant to growth and fermentation inhibitors (CROSBI ID 654709)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Štafa, Anamarija ; Pranklin, Andrea ; Zandona, Antonio ; Žunar, Bojan ; Svetec Miklenić, Marina ; Šantek, Božidar ; Svetec, Ivan Krešimir Construction of novel Saccharomyces cerevisiae hybrid strains resistant to growth and fermentation inhibitors // Journal of Biotechnology, Special Issue / Dundar, Munis (ur.). 2017. str. 15-15 doi: 10.1016/j.jbiotec.2017.06.054

Podaci o odgovornosti

Štafa, Anamarija ; Pranklin, Andrea ; Zandona, Antonio ; Žunar, Bojan ; Svetec Miklenić, Marina ; Šantek, Božidar ; Svetec, Ivan Krešimir

engleski

Construction of novel Saccharomyces cerevisiae hybrid strains resistant to growth and fermentation inhibitors

Bioethanol production from raw lignocellulosic material requires a producer strain which tolerates both the presence of growth and fermentation inhibitors and high ethanol concentrations. Therefore, we constructed heterozygous (hybrid) diploid S. cerevisiae strains by mating two haploid isolates having those desirable traits. In comparison to parental haploids, hybrid diploids were more resistant to several inhibitors frequently found in different lignocellulosic hydrolysates, such as acetic and levulinic acid and 2-furaldehyde. Interestingly, hybrids showed different fermentation ability in a CO2 production test. Our results suggest that construction of intraspecies hybrids coupled with the use of different genetic engineering techniques is a promising approach for the improvement or development of new biotechnologically relevant strains of S. cerevisiae. Additionally, we also show that in two biotechnologically interesting natural isolates percentage of successful gene targeting (gene targeting fidelity) is much lower than in widely used laboratory strains. Furthermore, the most frequent off-targeted event was duplication of a targeted chromosome yielding aneuploids having two copies of targeted chromosome, one containing transformed and the other containing untransformed allele. These results warn us that it could be more difficult to make precise genetic modifications of natural S. cerevisiae strains than expected.

yeast hybrids, growth and fermentation inhibitors

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Podaci o prilogu

15-15.

2017.

objavljeno

10.1016/j.jbiotec.2017.06.054

Podaci o matičnoj publikaciji

Journal of Biotechnology, Special Issue

Dundar, Munis

Podaci o skupu

European Biotechnology Congress 2017

pozvano predavanje

01.01.2017-01.01.2017

Dubrovnik, Hrvatska

Povezanost rada

Biotehnologija

Poveznice