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izvor podataka: crosbi

Exogenous isolation of antibiotic and metal resistance plasmids from pharmaceutical wastewaters released into the environment (CROSBI ID 654540)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Gonzalez Plaza, Juan Jose ; Blau, Khald ; Šimatović, Ana ; Milaković, Milena ; Bielen, Ana ; Ahel, Marijan ; Smalla, Kornelia ; Udiković Kolić, Nikolina Exogenous isolation of antibiotic and metal resistance plasmids from pharmaceutical wastewaters released into the environment // 4th THETheodor Escherich Symposium on Medical Microbiome Research : Abstract Book. 2017. str. 6-6

Podaci o odgovornosti

Gonzalez Plaza, Juan Jose ; Blau, Khald ; Šimatović, Ana ; Milaković, Milena ; Bielen, Ana ; Ahel, Marijan ; Smalla, Kornelia ; Udiković Kolić, Nikolina

engleski

Exogenous isolation of antibiotic and metal resistance plasmids from pharmaceutical wastewaters released into the environment

Antibiotic resistance (AR) is currently one of the most significant epidemiological risks. It is linked to anthropogenic activities, as release of pharmaceutical industry wastewaters, which often contain high concentrations of antibiotics and metals. Release into aquatic environment is assumed to promote dissemination of antibiotic resistance genes (ARGs) among environmental bacteria, and potentially to human pathogens. We need more information to understand the mobilization of ARGs in the environment influenced by antibiotic pollution to control the emergence of resistant pathogens. Studies of sediments at discharge points of two pharmaceutical factories in Croatia showed a high proportion of antibiotic resistant bacteria (ARB), which may lead to increased AR in human pathogens by horizontal gene transfer (HGT). Conjugation is one of most important HGT mechanisms for the spread of ARGs. Once established on plasmids, resistance genes may spread across different strains, species, or genera. Moreover, multiple ARGs are often co-localized on the same plasmid (coresistance), allowing for the spread of multidrug resistance. We have assayed whether plasmids containing ARGs genes from pharmaceutical discharge sites are transferable to other bacteria through exogenous plasmid transfer. Wastewater or sediments influenced by antibiotic pollution, as well as sediments from upstream and downstream sites, were mixed with model recipient E. coli. Transconjugants were selected based on AR markers and green fluorescent signal emission. Frequency of transconjugation was calculated for each site. Besides, we have characterized the presence/absence of ARGs as well as other metal/biocide resistance genes through qPCR and PCR, and antibiogram profiles for unique isolates were determined.

Exogenous plasmid transfer ; Horizontal gene transfer ; Antibiotic resistance

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Podaci o prilogu

6-6.

2017.

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objavljeno

Podaci o matičnoj publikaciji

4th THETheodor Escherich Symposium on Medical Microbiome Research : Abstract Book

Podaci o skupu

Theodor Escherich Symposium 2017

predavanje

12.10.2017-13.10.2017

Graz, Austrija

Povezanost rada

Interdisciplinarne prirodne znanosti