Naslov
Metallothionein (MT) expression and trace elements (TE) concentration in rat liver and kidney tissues ; sex differences and effect of gonadectomy

Autori
Orct, Tatjana ; Ljubojević, Marija ; Jurasović, Jasna ; Breljak, Davorka ; Vrhovac Madunić, Ivana ; Karaica, Dean ; Micek, Vedran ; Rašić, Dubravka ; Novak Jovanović, Ivana ; Peraica, Maja ; Gerić, Marko ; Gajski, Goran ; Nanić, Lucia ; Rubelj, Ivica ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts Joint 16th International Symposium on Trace Elements in Man and Animals (TEMA-16), 12th Conference of the International Society for Trace Element Research in Humans (ISTERH 2017) and 13th Conference of the Nordic Trace Element Society (NTES 2017) ; Journal of Trace Elements in Medicine and Biology 41 (2017) (S1) ; 1-88 / Skalnaya, Margarita G. ; Skalny, Anatoly V. ; Tinkov, Alexey A. - : Elsevier, 2017, 51-51

Skup
Joint 16th International Symposium on Trace Elements in Man and Animals (TEMA-16), 12th Conference of the International Society for Trace Element Research in Humans (ISTERH 2017) and 13th Conference of the Nordic Trace Element Society (NTES 2017)

Mjesto i datum
Sankt Peterburg, Rusija, 26.-29.6.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Metallothionein ; Trace elements ; Rat liver ; Rat kidney ; Gonadectomy

Sažetak
MT can bind essential (Zn, Fe, Cu, Co) and toxic (Cd, Hg) TE with different affinities. Sex-related expression of MT in specific organs and correlation with tissue concentration of TE have not been investigated. Here we studied in the liver (LV) and kidney (KD) tissues from adult sham-operated and gonadectomized male (M) and female (F) rats the expression of MT mRNA by RT-PCR and MT protein by immunochemical methods, and concentrations of the above-indicated TE. In both organs, MT mRNA and protein expression was strongly sex-related (F>M), weakly upregulated by castration, and strongly downregulated by ovariectomy. In control rats, similar pattern of sex-related TE concentrations (F>M) was found in LV for Cu, Fe, Cd, and in KD for Zn, Cu, Co, Fe, Hg, Cd, while Zn in LV was F<M. In gonadectomized rats, however, the pattern of TE concentrations in both organs was variable and poorly related to MT expression. Supported by HRZZ grant IP-11-2013-1481.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Javno zdravstvo i zdravstvena zaštita

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