engleski

LPMO as a key player in the enzyme conversion of biomass

Lignocellulose is a renewable resource that can be used for the sustainable production of platform chemicals or fuels. However, the recalcitrance of cellulose to hydrolytic depolymerization is a barrier to microbial and industrial utilization of lignocellulosic biomass. Cellulose is broken down to glucose by endo- and exo-acting glycosyl hydrolases which process the glucan chains in cellulose to soluble cellobiose moieties. In a process known as oxidative cellulose degradation, copper-dependent lytic polysaccharide monooxygenases (LPMO) cleave polymeric substrates, including crystalline cellulose, hemicelluloses and starch. It remains difficult to find how LPMOs orchestrate the complex reaction between copper reduction, co-substrate activation and substrate binding. To test the hypothesis that copper reduction increases binding of LPMO to cellulose, we studied the binding of oxidized and reduced LPMO to amorphous cellulose (PASC) and to microcrystalline cellulose (MC). Also, we aimed to quantify the substrate binding by measuring binding isotherms. The data demonstrate that copper reduction is a driver for substrate binding.

Lignocellulose, LPMO, copper reduction, substrate binding

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