engleski

Efect of age and follicle size on lipid profile and antioxidant potential of bovine follicular fluid

This study determined the lipid profle and antioxidative properties of bovine follicular fluid (FF) and also investigated their possible changes during foliculogenesis and reproductive ageing. Ovaries of heifers and cows were collected at a slaughterhouse. Samples of FF were obtained from follicles of three sizes: <5 mm (N=38), >5 mm (N=40) and >10 mm (N=35). Concentrations of triacylglycerol (TAG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), phospholipids (PHL) and activities of glutathione peroxidase (GPx), superoxide dismutase (SOD) and paraoxonase 1(PON 1) were analysed. The concentrations of TC, LDL- C, HDL- C, PHL were signifcantly higher, while the activities of GSH-Px and SOD were signifcantly lower in FF of cows compared to heifers. During foliculogenesis in the follicles of the heifers, concentrations of TAG, NEFA and the activity of SOD signifcantly decreased. Furthermore, during foliculogenesis in the follicles of the cows, concentrations of LDL-C and TAG as well as the activity of SOD signifcantly decreased, whereas concentration of TC signifcantly increased. In conclusion, these fndings indicate that age, together with the growth and development of follicles, affects antioxidant enzyme activity and lipid concentrations.

antioxidative status ; lipid profile ; bovine follicular fluid

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