engleski

Genotyping of the leptin receptor gene in Black Slavonian pig – preliminary results suggests new variants of the promoter

Researches on polymorphisms in the porcine LEPR gene and their association with economic traits were widely performed in the past. Manny polymorphisms in different part of the LEPR gene were described and majority of them was associated with economic traits such as growth and fatness. In present study, LEPR gene in 68 Black Slavonian pigs was genotyped for HinfI polymorphism in the 3, 8kb part of LEPR promoter, for HpaII and RsaI polymorphisms in the intron 4 and for ApeKI polymorphism in the exon 14. Allelic and genotype frequencies on polymorphic sites were calculated. Restriction of the 3.8 kb of the promoter region with HinfI revealed presence of two distinct restriction patterns, which haven’t been described so far. Their exact location and also their potential role in LEPR expression, as well as their impact on important economic traits should be explored in the future. Allelic and genotypic frequencies for other three polymorphic sites included in our study were more or less comparable with previous findings in the literature. Nevertheless, correlation of all investigated LEPR polymorphic sites with important performance and meat quality traits in Black Slavonian pigs is planned for the future.

Black Slavonian pig ; LEPR polymorphisms

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