Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 900709

Microscopic and molecular analysis of Babesia canis in archived and diagnostic specimens reveal the impact of anti-parasitic treatment and postmortem changes on pathogen detection


Huber, Doroteja; Beck, Ana; Anzulović, Željka; Jurković, Daria; Polkinghorne, Adam; Baneth, Gad; Beck, Relja
Microscopic and molecular analysis of Babesia canis in archived and diagnostic specimens reveal the impact of anti-parasitic treatment and postmortem changes on pathogen detection // Parasites & Vectors, 10 (2017), 495-495 doi:10.1186/s13071-017-2412-1 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 900709 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Microscopic and molecular analysis of Babesia canis in archived and diagnostic specimens reveal the impact of anti-parasitic treatment and postmortem changes on pathogen detection

Autori
Huber, Doroteja ; Beck, Ana ; Anzulović, Željka ; Jurković, Daria ; Polkinghorne, Adam ; Baneth, Gad ; Beck, Relja

Izvornik
Parasites & Vectors (1756-3305) 10 (2017); 495-495

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Babesia canis ; Merozoite morphology ; Postmortem cytological detection ; Influence of imidocarb dipropionate ; Genotyping

Sažetak
Background Classification of Babesia parasites has traditionally relied on morphological differentiation based on piroplasm size and shape. Molecular typing has subsequently revealed a more complex taxonomy for these piroplasms than previously thought. To evaluate the factors that influence the morphology of Babesia species upon microscopic examination and hence, their taxonomic classification, we performed detailed characterizations of piroplasms from archival and prospective collections of cytological samples of dogs with piroplasmosis before and after death. Merozoite morphology and time of parasite disappearance following imidocarb dipropionate was also investigated. Methods The study was divided into a (i) review of archived cytological slides from confirmed cases of canine piroplasmosis, and (ii) a prospective study of smears and tissue imprints from 15 recently necropsied dogs. The latter group could be further sub-divided into a non- treated group and an imidocarb dipropionate- treated group. Exact times of treatment before death were reviewed. Additional blood smears prepared from the live dogs and taken before therapy were also evaluated in the latter group. Parasite burden per each slide was determined in both studies. The shape and size of merozoites were described from blood smears taken while the dogs were alive and from different organs during necropsy. The results of all measurements were statistically analyzed. Results The morphology and size of merozoites from live dogs corresponded to that of previously described ‘large’ Babesia. The morphology and size of merozoites were significantly different (P < 0.001) in postmortem samples, however, and more consistent in shape and size with piroplasm cells previously referred to as ‘small’ Babesia. PCR and sequencing confirmed B. canis as the causative agent of disease in all investigated dogs, including in postmortem negative tissue imprints from dogs treated at least 24 h before death. Conclusions Changes in the morphology of ‘large’ B. canis to ‘small’- like Babesia observed by light microscopy appear to represent a common postmortem change. Classification of Babesia parasites into ‘large’ and ‘small’ Babesia using only microscopy of postmortem slides should be treated with caution. PCR-based methodologies for detection and molecular typing of Babesia spp. may prove valuable for investigating suspected cases of babesiosis following necropsy.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
GENOTICKTRACK - projekt HRZZ br. 1957 - GENOTICKTRACK - projekt HRZZ br. 1957 (, )

Ustanove
Hrvatski veterinarski institut, Zagreb,
Veterinarski fakultet, Zagreb

Profili:

Avatar Url Daria Jurković (autor)

Avatar Url Relja Beck (autor)

Avatar Url Doroteja Huber (autor)

Avatar Url Ana Beck (autor)

Citiraj ovu publikaciju

Huber, Doroteja; Beck, Ana; Anzulović, Željka; Jurković, Daria; Polkinghorne, Adam; Baneth, Gad; Beck, Relja
Microscopic and molecular analysis of Babesia canis in archived and diagnostic specimens reveal the impact of anti-parasitic treatment and postmortem changes on pathogen detection // Parasites & Vectors, 10 (2017), 495-495 doi:10.1186/s13071-017-2412-1 (međunarodna recenzija, članak, znanstveni)
Huber, D., Beck, A., Anzulović, Ž., Jurković, D., Polkinghorne, A., Baneth, G. & Beck, R. (2017) Microscopic and molecular analysis of Babesia canis in archived and diagnostic specimens reveal the impact of anti-parasitic treatment and postmortem changes on pathogen detection. Parasites & Vectors, 10, 495-495 doi:10.1186/s13071-017-2412-1.
@article{article, year = {2017}, pages = {495-495}, DOI = {10.1186/s13071-017-2412-1}, keywords = {Babesia canis, Merozoite morphology, Postmortem cytological detection, Influence of imidocarb dipropionate, Genotyping}, journal = {Parasites and Vectors}, doi = {10.1186/s13071-017-2412-1}, volume = {10}, issn = {1756-3305}, title = {Microscopic and molecular analysis of Babesia canis in archived and diagnostic specimens reveal the impact of anti-parasitic treatment and postmortem changes on pathogen detection}, keyword = {Babesia canis, Merozoite morphology, Postmortem cytological detection, Influence of imidocarb dipropionate, Genotyping} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • CAB Abstracts
  • CA Search (Chemical Abstracts)
  • EMBASE (Excerpta Medica)
  • MEDLINE
  • DOAJ
  • Global Health
  • PubMed
  • PubMed Central
  • Science Citation Index Expanded
  • SCImago
  • ​Scopus


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font