engleski

Mixoploidy and chimeric structures in somaclones of potato (Solanum tuberosum L.) cv. Bintje

Plant tissue culture is recognised as a disposable tool to generate useful genetic variability for crop improvement. Nine somaclones of tetraloid potato cv. Bintje regenerated from stem, tuber or protoplast-derived callus were analysed in vitro and in the field. All of them were morphological and cytological chimeras. They had alterations in general appearance, leaf morphology and tuber characteristics. The phenotypic variations of the first vegetative progeny (SC2 plants) were in most cases different in comparison with the primary regenerants (SC1 plants). The observed phenotypic instability was in correlation with chromosome mosaicism (chromosomal instability) as aneuploid and polyploid cells were detected at high frequency (57-89%) in root tips of all somaclons investigated.

Solanum tuberosum L. potato; callus culture; regenerants; somaclonal variation; chimeras; mixoploidy

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