engleski

Recent trends in application of virus-derived particles as nucleic acid delivery agents

Nucleic acid delivery comprises vector mediated transfer of genetic material into cells and favourable alternation of cellular function or structure. Considering the intolerance of cells toward exogenous genetic material, the vectors of interest have to act on different steps along the gene expression pathway in order to evoke the desired outcome. In the last 25 years of gene/cell therapy, viral vectors have dominated clinical trials due to their superior transduction efficiency acquired through evolution. Non-viral vectors involve a variety of (bio)chemical particles that are generally less efficient than viruses, but their application is comparatively less costly and with reduced adverse effects on patients and biosafety. Arelativelynovelcategoryofnon- viraltransfectionagentsincludes derivatives of viral peptides that owing to their origin can mimic viral strategies in cell targeting and nucleic acid delivery. The potentialadvantageofthesebiomolecularparticlesl iesincomplementary blend of viral efficiency and chemical particle tractability suitable for design of highly effective and safe gene therapy vehicles. This may be achieved by using standard molecular biology techniques as well as in vitro chemical modification in order to improve the bio-particles’ composition and mode of action.

transfection, viral vectors

Rad je sufinanciran sredstvima HRZZ projekta 2016- 06-3848