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Comparative analysis of growth, development and OTC production of various S. rimosus producers


Paradžik, Tina; Šarić, Ela; Quinn, Gerre; Roje, Marin; Jurin, Mladenka; Hunter, Iain; Herron, Paul; Vujaklija, Dušica
Comparative analysis of growth, development and OTC production of various S. rimosus producers // Programme book of 7th Congress of European Microbiologists
Valencija, Španjolska, 2017. (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Comparative analysis of growth, development and OTC production of various S. rimosus producers

Autori
Paradžik, Tina ; Šarić, Ela ; Quinn, Gerre ; Roje, Marin ; Jurin, Mladenka ; Hunter, Iain ; Herron, Paul ; Vujaklija, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Programme book of 7th Congress of European Microbiologists / - , 2017

Skup
FEMS 2017, 7th Congress of European Microbiologists

Mjesto i datum
Valencija, Španjolska, 09.-13.07.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Phosphorylation, S. rimosus, OTC production

Sažetak
Streptomycetes are multicellular bacteria that exhibit a complex developmental program and morphological differentiation. The great majority of natural products, including clinnicaly important antibiotics, immunosuppressants and anti-cancer drugs ; are synthesized by these bacteria. The tetracyclines are one of the most successful classes of antibiotics that include oxytetracycline (OTC) produced by S. rimosus. It has been reported that protein phosphorylation has an important role in the regulation of metabolism and antibiotic production in strepomycetes. Bioinformatic analysis of the S. coelicolor genome predicted 47 eukaryotic-like protein kinases and 49 eukaryotic type protein phosphatases in this species. This number of kinases, although larger than other bacteria is not surprising as the average Streptomyces genome size is almost twice that of E.coli. Based on the phosphoproteome reported for S. coelicolor we predict that the effect of the OTC overproduction in S. rimosus is a consequence of alteration in posttranslational modification (PTM) of regulatory and/or OTC biosynthesis proteins. Different S. rimosus strains were tested for antibiotic production, and analysed using HPLC. Level of phosphorylation during growh was inspected by Western blot and morphology was observed applying viability staining to each strain. Inspected strains showed great variations in OTC production. Western blot analyses showed different phosphorylation patterns for each strain. Confocal microscopy revealed different morphological features and viability of tested strains.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb