Comparative analysis of growth, development and OTC production of various S. rimosus producers (CROSBI ID 653260)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Paradžik, Tina ; Šarić, Ela ; Quinn, Gerre ; Roje, Marin ; Jurin, Mladenka ; Hunter, Iain ; Herron, Paul ; Vujaklija, Dušica
engleski
Comparative analysis of growth, development and OTC production of various S. rimosus producers
Streptomycetes are multicellular bacteria that exhibit a complex developmental program and morphological differentiation. The great majority of natural products, including clinnicaly important antibiotics, immunosuppressants and anti-cancer drugs ; are synthesized by these bacteria. The tetracyclines are one of the most successful classes of antibiotics that include oxytetracycline (OTC) produced by S. rimosus. It has been reported that protein phosphorylation has an important role in the regulation of metabolism and antibiotic production in strepomycetes. Bioinformatic analysis of the S. coelicolor genome predicted 47 eukaryotic-like protein kinases and 49 eukaryotic type protein phosphatases in this species. This number of kinases, although larger than other bacteria is not surprising as the average Streptomyces genome size is almost twice that of E.coli. Based on the phosphoproteome reported for S. coelicolor we predict that the effect of the OTC overproduction in S. rimosus is a consequence of alteration in posttranslational modification (PTM) of regulatory and/or OTC biosynthesis proteins. Different S. rimosus strains were tested for antibiotic production, and analysed using HPLC. Level of phosphorylation during growh was inspected by Western blot and morphology was observed applying viability staining to each strain. Inspected strains showed great variations in OTC production. Western blot analyses showed different phosphorylation patterns for each strain. Confocal microscopy revealed different morphological features and viability of tested strains.
phosphorylation ; S. rimosus ; OTC production
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Podaci o prilogu
FEMS7-2146
2017.
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objavljeno
Podaci o matičnoj publikaciji
FEMS 2017, 7th Congress of European Microbiologists : Book of abstracts
Podaci o skupu
FEMS 2017, 7th Congress of European Microbiologists
poster
09.07.2017-13.07.2017
Valencia, Španjolska