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Supercritical CO2 extraction of Salvia officinalis L. leaves targeted on oxygenated monoterpenes, α-humulene, viridiflorol, and manool

A comprehensive approach was applied to optimize the supercritical CO2 (SC-CO2) extraction conditions of Salvia officinalis L. (sage) leaves. Different extraction parameters (pressure 10-30 MPa, temperature 40-60 °C, and CO2 flow rate 1-3 kg/h) were investigated: The extraction yield, the extracts' chemical profile (determined by GC-MS), and the optimal extraction conditions by response surface methodology (RSM) for the targeted most abundant components. Within the range of the applied extraction parameters, the major compounds of all SC-CO2 extracts were oxygenated monoterpenes, namely, 1, 8-cineole (6.56-27.26 mg CE/g), α-/β-thujone (11.56-44.82 mg CE/g), and camphor (38.23- 104.71 mg CE/g), followed by α-humulene (39.90- 90.73 mg/g), viridiflorol (48.07-97.01 mg CE/g), and manool (113.90-335.36 mg CE/g). The pressure exhibited the most significant influence on the yield (0.242-7.361 mass %) and significantly influenced the amount of 1, 8- cineole, α-/β-thujone, and camphor (within the range 15 or 20 MPa). In contrast, the highest amount of α-humulene, viridiflorol, and manool was obtained at the lowest pressure (10 MPa). The results showed that that the second order polynomial model can be effectively used for the optimization and prediction of targeted compounds.

Salvia officinalis L. ; supercritical CO2 extraction ; GC-MS ; response surface methodology

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