engleski

Antimicrobial susceptibilities of Aeromonas species isolated from fish in Croatian rivers

The genus Aeromonas consists of Gram-negative rods, ubiquitous in the aquatic environment and are common constituents of the microbial community of fish. However, they are also capable of causing serious infections, as opportunistic pathogens in humans and as a serious economic threat to fish farming worldwide. Occurrence of the antibiotic- resistant bacteria is increasing in the aquatic environments. The problem has become more serious when inappropriate use of antibiotics causes an unnecessary impact on the environment and development of resistant bacteria, thereby reducing the efficacy of antibiotic treatment of diseases. The aim of this study was to investigate antimicrobial susceptibility profiles of Aeromonas species isolated from fish in Croatian rivers. A total of 32 fish were collected from the rivers: Dobra, Kupa and Mrežnica for the isolation of Aeromonas. Isolates were obtained onto Tryptic soy agar and identified using MALDI TOF spectrometry. Identified Aeromonas were represented as A. caviae, A. ichtiosmia, A. sobria and A. veronii. Fifteen antibacterial agents were used for in vitro sensitivity testing of 32 selected isolates by disc diffusion method. All Aeromonas isolates were susceptible to nitrofurantion, norfloxacin, ofloxacin, floron and flumequine. All isolates were resistant to novobiocin, ampicilin and penicilin. Resistance to antibiotics may be strain dependent, but variations of resistance to some antibiotics has occurred between isolates of the same Aeromonas species within the same river, and it may become a problem and a sign of changes of resistance.

antibiotic-resistance ; fish microbiota ; Aeromonas ; pathogens ; fish farming

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