engleski

Post-ischemic administration of erythropoietin: effects on the brain damage in rats exposed to focal cerebral ischemia

Introduction: The purpose of our study was to investigate the influence of recombinant human erythropoietin (rhEpo) on the brain infarct volume and neuronal degeneration in the entorhinal cortex of rats exposed to the right middle cerebral artery occlusion (MCAO). Materials and methods: Male Hannover Wistar rats were exposed to the focal cerebral ischemia by right MCAO for 1 hr. Sham operated, vehicle treated animals served as the control group. Ischemic animals received either vehicle or rhEpo (5000 IU/kg, i.p.), 3 h after induction of ischemia. Rats were sacrificed 24 h after the onset of the ischemic or sham experimental procedure. The extent of the brain infarct volume was determined by using the TTC dye at 24 h after induction of MCAO procedure in ischemic, vehicle treated animals such as in ischemic animals treated with rhEpo. Fluoro-Jade B histofluorescence was used for the evaluation of entorhinal cortical neurons undergoing neurodegenerative changes and immunohistochemical detection of NeuN for neuronal loss measurement. Results: Our results showed that MCAO/reperfusion cause marked cortical infarction. In addition, after MCAO, prominent Fluoro-Jade B staining and the decrease in NeuN immunoreactivity were detected. Post-ischemic administration of rhEpo significantly reduced the brain infarct volume, neuronal degeneration and neuronal loss in the ischemic animals exposed to MCAO in comparison to the rats of the control group. Conclusion: Our results suggest that post-ischemic administration of rhEpo exerts a neuroprotective potential in rats exposed to focal cerebral ischemia.

recombinant human erythropoietin, entorhinal cortex, right middle cerebral artery occlusion, rat

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