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TDP-43 expression in the parietal cortex and the hippocampus following a single traumatic brain injury in the mouse

TAR DNA-binding protein-43 (TDP-43) is recognized as one of the main disease-associated proteins in many neurodegenerative diseases and recent data indicate that it also could be included in complex cascade of different pathophysiological processes initiated by traumatic brain injury (TBI). The aim of this study was to investigate the intracellular localizations of the TDP-43 in different types of the brain cells in the parietal cortex and the hippocampus in experimental TBI. Single moderate lateral fluid percussion injury (LFPI) was performed over the left parietal cortex in adult male C57BL/6 mice. Animals were sacrificed 3 or 14 days after the LFPI and their brains were prepared for immunohistological analyses. Sham-operated animals were used as the control group. Brain slices were double-stained with TDP-43 antibody together with antibodies against specific neuronal, astrocytic or microglial markers. TDP-43 nuclear expression was detected in all mentioned cell types of both investigated brain structures in all injured animals. TDP-43 cytoplasmatic locatization was found in the neurons and the microglia of the parietal cortex at 3 and 14 days after TBI, while no such changes were observed in the hippocampus. Our preliminary study suggests that even a single moderate TBI can be associated with the changes in the intracellular localization of the TDP-43 immunoreactivity, in the neurons and the microglial cells of the parietal cortex in the mouse.

TDP-43, parietal cortex, hippocampus, traumatic brain injury, mouse

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