engleski

Lateral fluid percussion injury induces astrocytosis and microgliosis without a neuronal loss in the rat frontal cortex

Traumatic brain injury (TBI) affects many brain regions but those more distant to the impact site have been poorly studied. Therefore, the purpose of this research was to determine the changes of the neurons, astrocytes and microglial cells in the frontal cortex at different time points following experimental TBI. TBI of moderate severity was induced over the left parietal cortex in adult male Wistar rats by using the lateral fluid percussion injury (LFPI) method. Sham-operated animals were used as the control group. Rats were sacrificed 1, 3 or 7 days after the injury or sham procedure and their brains were prepared for the immunohistological analyses. NeuN, GFAP and Iba1 antibodies were used as the markers for neurons, astrocytes and microglial cells, respectively. Significant astrocytosis and microgliosis were detected at day 7 after the injury while the number of neurons in the rat frontal cortex was not significantly changed in any of the investigated time points after the brain trauma. Our study showed marked inflammatory response and no neuronal loss in the frontal cortex, the brain region distant from the primary impact site, in the first week following the LFPI in the rat.

inflammation, lateral fluid percussion injury, frontal cortex, neuronal loss, rat

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