Isolation and identification of Yersinia enterocolitica strains from food production chain (CROSBI ID 652858)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Zdolec, Nevijo ; Dobranić, Vesna ; Kazazić, Snježana ; Šimunić, Ivan ; Dumbović, Zvonimir
engleski
Isolation and identification of Yersinia enterocolitica strains from food production chain
Human foodborne yersiniosis is mostly associated with consumption of raw or undercooked food of animal origin. During a three-year period, we tested 91 thermally processed and fermented meat products, 32 fresh meat and meat preparations at the end of their shelf-life, 295 raw milk and 9 unpasteurized milk cheeses sampled at retail level, and 78 pig tonsils sampled during slaughter processing, for the presence of Yersinia enterocolitica. Samples were enriched in PSB and ITC broth, and then streaked on CIN agar and CHROMagarTM Y.enterocolitica. Presumptive colonies were identified by MALDI-TOF MS. Pathogenic Y. enterocolitica was absent in raw milk, unpasteurized milk cheeses, thermally processed and fermented meat products. Y. enterocolitica was recovered from 26 tonsils (33.33 %) and two fresh pork meat samples from retail (6.25 %). A total of 80 presumptive colonies were selected from CIN/CHROMAgar and all were identified as Y. enterocolitica by means of MALDI-TOF MS. The results demonstrate the low occurrence of pathogenic Y. enterocolitica in dairy products and processed meats on the market. On the other hand, the persistence of pathogen in the pig population is a risk factor for meat contamination during slaughter processing and meat cutting. The combination of chromogenic culture media and MALDI -TOF MS may be used as alternative method for faster isolation and confirmation of pathogenic Y. enterocolitica from food chain samples.
Yersinia enterocolitica ; food chain ; MALDI-TOF MS ; chromogenic media
Istraživanje je financiralo Sveučilište u Zagrebu, kratkoročna potpora istraživanju "Izolacija i karakterizacija patogenih Yersinia enterocolitica u lancu proizvodnje domaćih i divljih svinja" (voditelj: Nevijo Zdolec).
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Podaci o prilogu
99-99.
2017.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts of 7th International Congress "Veterinary Science and Profesion"
Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran
Zagreb: Veterinarski fakultet Sveučilišta u Zagrebu
978-953-8006-13-5
Podaci o skupu
7th International Congress "Veterinary Science and Profesion"
poster
05.10.2017-07.10.2017
Zagreb, Hrvatska