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Pregled bibliografske jedinice broj: 897133

Interaction of secondary metabolites from cyanobacterial strains with cellular detoxification mechanisms as determined by effects-directed analyses


Marić, Petra; Ahel, Marijan; Babić, Olivera; Simeunović, Jelica; Smital, Tvrtko
Interaction of secondary metabolites from cyanobacterial strains with cellular detoxification mechanisms as determined by effects-directed analyses // Chemosphere (2017) (znanstveni, poslan)


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Naslov
Interaction of secondary metabolites from cyanobacterial strains with cellular detoxification mechanisms as determined by effects-directed analyses

Autori
Marić, Petra ; Ahel, Marijan ; Babić, Olivera ; Simeunović, Jelica ; Smital, Tvrtko

Vrsta, podvrsta
Radovi u časopisima, znanstveni

Izvornik
Chemosphere (2017)

Status rada
Poslan

Ključne riječi
cyanobacterial strains ; secondary metabolites ; effects-directed analyses ; cellular detoxification pathway ; in vitro bioassays ; drOatp1d1/drOct1 ; CYP1A1

Sažetak
Cyanobacterial strains produce various toxic secondary metabolites that represent a significant threat to the environment and human health, especially during periods of intensive blooms. Effects-directed analyses (EDA) is a modern multidisciplinary diagnostic tool developed in the field of environmental science that combines the use of advanced chemical and biological methods in order to identify, characterize and prioritize toxicants present in complex environmental samples. In order to improve the present portfolio of EDA methodology we applied a modified EDA procedure based on a series of in vitro or small-scale bioassays in combination with advanced chemical analytical protocols. Main goal of this study was to identify molecular mechanism of cyanobacterial toxicity in extracts of selected cyanobacterial strains found in toxic cyanobacterial blooms in Vojvodina region, Serbia. Non-selective and non-target preparation techniques were used for extraction of a broad range of chemical compounds present in cyanobacterial strains. Ecotoxicological endpoints that were monitored in this study included acute and chronic toxicity, as well as interaction with basic phases of cellular detoxification: phase 0 uptake transport proteins, phase I and II detoxification enzymes, and phase III efflux transport proteins. Three terrestrial (Anabaena Č2, Anabaena Č5, Nostoc S8) and three aquatic (Nostoc Z1, Phormidium Z2, Oscillatoria K3) cyanobacterial strains were used in our modified EDA for detailed ecotoxicological characterization. All tested cyanobacterial strains are found to be chronically cytotoxic. Mechanisms of toxicity of secondary metabolites from cyanobacterial strains include interaction with the phase 0 and I of the cellular detoxification pathway, especially inhibition of zebrafish Oatp1d1 and/or Oct1 transporters. Aquatic cyanobacterial strains are considered to be of greater ecotoxicological importance. Oscillatoria K3 showed to be the most the interesting aquatic cyanobacterial strain because it was the most responsive strain to several observed biological effects.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-4806 - Identifikacija i funkcionalna karakterizacija (eko)toksikološki važnih polispecifičnih membranskih transportnih proteina u zebrici (Danio rerio) (TRANS-ZEBRATOX) (Smital, Tvrtko, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Marijan Ahel (autor)

Avatar Url Petra Marić (autor)

Avatar Url Tvrtko Smital (autor)


Citiraj ovu publikaciju

Marić, Petra; Ahel, Marijan; Babić, Olivera; Simeunović, Jelica; Smital, Tvrtko
Interaction of secondary metabolites from cyanobacterial strains with cellular detoxification mechanisms as determined by effects-directed analyses // Chemosphere (2017) (znanstveni, poslan)
Marić, P., Ahel, M., Babić, O., Simeunović, J. & Smital, T. (2017) Interaction of secondary metabolites from cyanobacterial strains with cellular detoxification mechanisms as determined by effects-directed analyses. Poslan u Chemosphere. [Preprint].
@unknown{unknown, year = {2017}, keywords = {cyanobacterial strains, secondary metabolites, effects-directed analyses, cellular detoxification pathway, in vitro bioassays, drOatp1d1/drOct1, CYP1A1}, journal = {Chemosphere}, title = {Interaction of secondary metabolites from cyanobacterial strains with cellular detoxification mechanisms as determined by effects-directed analyses}, keyword = {cyanobacterial strains, secondary metabolites, effects-directed analyses, cellular detoxification pathway, in vitro bioassays, drOatp1d1/drOct1, CYP1A1} }
@unknown{unknown, year = {2017}, keywords = {cyanobacterial strains, secondary metabolites, effects-directed analyses, cellular detoxification pathway, in vitro bioassays, drOatp1d1/drOct1, CYP1A1}, journal = {Chemosphere}, title = {Interaction of secondary metabolites from cyanobacterial strains with cellular detoxification mechanisms as determined by effects-directed analyses}, keyword = {cyanobacterial strains, secondary metabolites, effects-directed analyses, cellular detoxification pathway, in vitro bioassays, drOatp1d1/drOct1, CYP1A1} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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