hrvatski

Ekspresija tirozin kinaznih receptora epidermalnog čimbenika rasta u sporadičnom miksomu srca

AIMS: The epidermal growth factor receptor family (EGFR) consist of four tyrosine kinas receptors, EGFR1/c-erbB1, c-erbB2, cerbB3 and cerbB4. They are involved in tumor cell proliferation, differentiation, survival and angiogenesis. Co-expressed in the same neoplastic cell source with accompanying hetero-dimerization they further enhance pathologically important signaling transduction pathways. In the presented study, we analyzed the expression and cellular distribution of EGFR family members in sporadic cardiac myxoma lesions of human heart. METHODS AND RESULTS: The study included 36 patients, who were treated with surgical excision of the lesion. Expression of EGFR1/c-erbB1, c-erbB2, cerbB3 and cerbB4 was studied by immunohistochemical staining and qRT-PCR. The patient population was comprised of 27 (75%) women and 9 (25%) men. The mean age of patients were 63 years, with an age range of 34 to 87 years. All studied cases were sporadic myxoma originating in the left atrium. Majority of examined cases demonstrate moderate-to-strong and overlapping expression of EGFR family protein members in all commonly present histological types of neoplastic myxoma cells except the c-erbb2 which was immunohistochemically negative (HercepTest) in all cases. c-erbB4 showed statistically significant decrease in expression on mRNA level compared to normal left atrial tissue. Compared relative quantities of EGFR protein members with each other showed that c-erbB3 and c-erbB2 were the only ones exhibiting statistically significant (p=0.01304), and positive (r=0.721) correlation. CONCLUSION: EGFR1/c-erbB1, cerbB2 (mRNA only) cerbB3 and cerbB4 are co-expressed in the tissue of cardiac myxoma lesions and may play a distinct role in their pathogenesis.

miksom srca, erbb1 ; erbb2, erbb3 ; erbb4, koekspresija ; lijevi atrij ; sporadične lezije

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