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Peptide-Based Probes with an Artificial Anion Binding Motif for Direct Fluorescence 'Switch-On' Detection of Nucleic Acid in Cells


Maity, Debabrata; Matković, Marija; Li, Shang; Ehlers, Martin; Wu, Junchen; Piantanida, Ivo; Schmuck, Carsten
Peptide-Based Probes with an Artificial Anion Binding Motif for Direct Fluorescence 'Switch-On' Detection of Nucleic Acid in Cells // Chemistry : a European journal, 23 (2017), 68; 17356-17362 doi:10.1002/chem.201703813 (međunarodna recenzija, članak, znanstveni)


Naslov
Peptide-Based Probes with an Artificial Anion Binding Motif for Direct Fluorescence 'Switch-On' Detection of Nucleic Acid in Cells

Autori
Maity, Debabrata ; Matković, Marija ; Li, Shang ; Ehlers, Martin ; Wu, Junchen ; Piantanida, Ivo ; Schmuck, Carsten

Izvornik
Chemistry : a European journal (0947-6539) 23 (2017), 68; 17356-17362

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Molecular recognition ; fluorescent probe ; DNA detection ; cell imaging ; anion receptors

Sažetak
We report two new peptide based fluorescence probes 1 and 2 for the detection of ds-DNA at physiological pH. 1 and 2 contain amino-naphthalimide and diethyl-aminocoumarin fluorophores, respectively, with two identical peptide arms each equipped with a guanidiniocarbonylpyrrole (GCP) artificial anion-binding motif. 1 and 2 show ‘switch-on’ fluorescence response upon binding to ds-DNA, whereby they can differentiate between various types of polynucleotides. For instance they exhibit more pronounced fluorescence response for AT-rich polynucleotides than GC-rich polynucleotides, and both give only negligible response to ds-RNA. The fluorimetric response of 1 is proportional to the AT-basepair content in DNA, while the fluorescence of 2 is sensitive to secondary structure of polynucleotide. Fluorescence experiments, thermal melting experiments and circular dichroism studies reveal that 1 interacts with ds-DNA in a combined intercalation and minor groove binding, while 2 interacts mainly with the outer surface of DNA/RNA. As 1 and 2 have a very low cytotoxicity, 1 can be applied for the imaging of nuclear DNA in cells.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-1477 - Višenamjensko očitavanje DNA / RNA sekundarne strukture molekularnim kemijskim senzorima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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