Methylation-associated silencing of SFRP1 gene in high-grade serous ovarian carcinomas (CROSBI ID 242476)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Kardum, Vedran ; Karin, Valentina ; Glibo, Mislav ; Škrtić, Anita ; Nikuševa Martić, Tamara ; Ibišević, Nermina ; Skenderi, Faruk ; Vranićc, Semir ; Šerman, Ljiljana
engleski
Methylation-associated silencing of SFRP1 gene in high-grade serous ovarian carcinomas
Wnt is a highly conserved signaling pathway responsible for tissue regeneration, maintenance and differentiation of stem cells in adults. Its aberrant activation through reduced expression of Wnt signaling pathway inhibitors, such as proteins from the SFRP family, is commonly seen in many tumors. In the present study we explored SFRP1 protein expression using immunohistochemistry in 11 low-grade serous ovarian carcinomas (LGSC), 42 high-grade serous ovarian carcinomas (HGSC), and 5 normal ovarian tissues (controls). SFRP1 gene methylation was analyzed by methylation-specific PCR in 8 LGSCs, 13 HGSCs and control samples. SFRP1 gene was unmethylated and SFRP1 protein expression was strong in normal ovaries (n =5). Although SFRP1 gene was unmethylated in almost all of the LGSC cases (7/8, 88%), SFRP1 protein expression was significantly lower than in normal ovaries (p < 0.05). Seven out of 13 HGSCs (54%) showed SFRP1 gene hypermethylation and protein expression level was also significantly lower than in normal ovaries (p < 0.001). Our preliminary data show loss of SFRP1 protein expression caused by the SFRP1 promoter hypermethylation in a subset of HGSCs. SFRP1 protein expression was also lost in LGSCs but different regulatory mechanisms may be involved. Further studies should elucidate the clinical and therapeutic relevance of the observed molecular alterations.
Wnt pathway
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
31
2017.
45-49
objavljeno
1092-9134
1532-8198
10.1016/j.anndiagpath.2017.07.002
Povezanost rada
Temeljne medicinske znanosti