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Pregled bibliografske jedinice broj: 893049

New series of non-toxic DNA intercalators, mitochondria targeting fluorescent dyes


Kurutos, Atanas; Orehovec, Iva; Tomašić Paić, Ana; Crnolatac, Ivo; Horvat, Lucija; Gadjev, Nikolai; Piantanida, Ivo; Deligeorgiev, Todor
New series of non-toxic DNA intercalators, mitochondria targeting fluorescent dyes // Dyes and pigments, 148 (2018), 452-459 doi:10.1016/j.dyepig.2017.09.049 (međunarodna recenzija, članak, znanstveni)


Naslov
New series of non-toxic DNA intercalators, mitochondria targeting fluorescent dyes

Autori
Kurutos, Atanas ; Orehovec, Iva ; Tomašić Paić, Ana ; Crnolatac, Ivo ; Horvat, Lucija ; Gadjev, Nikolai ; Piantanida, Ivo ; Deligeorgiev, Todor

Izvornik
Dyes and pigments (0143-7208) 148 (2018); 452-459

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Monomethine cyanine dyes ; DNA/RNA ; ITC ; circular dichroism ; binding parameters ; mitochondria

Sažetak
Five novel benzoxazolium and benzothiazolium derived asymmetric dicationic monomethine cyanine dyes, bearing a thioester group have been synthesised via condensation of quaternary nitrogen containing heterocyclic salts in various anhydrides. Their chemical structures were evaluated by NMR, ESI/MS, IR spectroscopy, and melting point temperatures. Photophysical studies on the title compounds were performed in methanol solutions. The longest wavelength absorption maxima λmax of the studied dyes are in the region 453-508 nm. The corresponding fluorescence maxima λfl were registered within the 489-553 nm. Further spectroscopy and calorimetric techniques including circular dichroism (CD), UV-Vis, fluorescence, thermal denaturation and isothermal titration calorimetry have been applied to the studied dyes in the presence of biopolymers, in order to evaluate their staining ability as fluorescent markers. In aqueous solution the compounds exhibit no intrinsic fluorescence, however upon binding to dsDNA and dsRNA they show considerable enhancement of fluorescence intensity. Binding constants Ks of the complexes [dye–polynucleotide] have been evaluated from fluorescence spectral titrations. Although the intercalation is the predominant binding mode, the compounds show little or no effect on the cell proliferation. Upon fast cell internalization the compounds accumulate in the mitochondrial space.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekt / tema
HR.3.2.01 0254
HRZZ-IP-2013-11-1477 - Višenamjensko očitavanje DNA / RNA sekundarne strukture molekularnim kemijskim senzorima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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