Napredna pretraga

Pregled bibliografske jedinice broj: 892856

Functional transcripts indicate phylogenetically diverse active ammonia-scavenging microbiota in sympatric sponges


(6th China-Croatia Science and Technology cooperation committee program (No. 6–13)) Guofang, Feng; Wei, Sun; Fengli, Zhang; Orlić, Sandi; Zhiyong, Li
Functional transcripts indicate phylogenetically diverse active ammonia-scavenging microbiota in sympatric sponges // Marine biotechnology, 20 (2018), 2; 131-143 doi:10.1007/s10126-018-9797-5 (međunarodna recenzija, članak, znanstveni)


Naslov
Functional transcripts indicate phylogenetically diverse active ammonia-scavenging microbiota in sympatric sponges

Autori
Guofang, Feng ; Wei, Sun ; Fengli, Zhang ; Orlić, Sandi ; Zhiyong, Li

Kolaboracija
6th China-Croatia Science and Technology cooperation committee program (No. 6–13)

Izvornik
Marine biotechnology (1436-2228) 20 (2018), 2; 131-143

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Sponge ; ammonia-oxidizing archaea ; ammonia-assimilating bacteria ; functional transcript ; RT-qPCR assays

Sažetak
Symbiotic ammonia-scavengers contribute to effective removal of ammonia in sponges. However, the phylogenetic diversity and in situ activity of ammonia-scavenging microbiota between different sponge species are poorly addressed. Here, transcribed ammonia monooxygenase genes (amoA), hydrazine synthase genes (hzsA) and glutamine synthetase genes (glnA) were analyzed to reveal the active ammonia-scavenging microbiota in the sympatric sponges Theonella swinhoei, Plakortis simplex, Phakellia fusca, and seawater. Archaeal amoA and bacterial glnA transcripts rather than bacterial amoA, hzsA, and archaeal glnA transcripts were detected in the investigated sponges and seawater. The transcribed amoA genes were ascribed to two Thaumarchaeota ecotypes, while the transcribed glnA genes were interspersed among the lineages of Cyanobacteria, Tectomicrobia, Poribacteria, Alpha-, Beta-, Gamma- and Epsilonproteobacteria. In addition, transcribed abundances of archaeal amoA and bacterial glnA genes in these sponges have been quantified, showing significant variation among the investigated sponges and seawater. The transcriptome-based qualitative and quantitative analyses clarified the different phylogenetic diversity and transcription expression of functional genes related to microbially- mediated ammonia scavenging in different sympatric sponges, contributing to the understanding of in situ active ecological functions of sponge microbial symbionts in holobiont nitrogen cycling.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Sandi Orlić, (245206)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati