engleski

Effect of additive Lactobacillus plantarum on ammonium production in whole plant corn silage during prolonged storage

Ensiling is a method for moist forage preservation based on natural fermentation of sugars to acids, mainly lactic acid. Lactobacillus plantarum is often used as additive during ensiling to promote optimal fermentation resulting in rapid lactic acid production and pH drop thus improving dominant nutrients preservation. Ammonium production is simple indicator of protein degradation and deamination in silages. Literature states proteolysis is active primarily during first few weeks of silage production. Excess proteolysis in silages is connected with multiple negative effects on silage quality and animal performance. In the present study, the application of Lactobacillus plantarum on ammonium production in WPCS during prolonged storage of silage was investigated. Yellow corn hybrid (Bc 462) was ensiled in five replications in laboratory scale silos without and with Lactobacillus plantarum additive (300000 CFU/g fresh material). Silages were sampled at the beginning and on 15th, 48th, 98th, 182th, 274th and 364th day of ensiling when contents of ammonium (g/kg of CP), crude protein (CP) and DM were monitored. Effects of time, silage additive and their interactions on silages were tested using the PROC MIXED procedure in SAS 9.3. The analyses showed that the ammonium contents increased in all silages during whole period of prolonged storage (P<0, 05) and were under influence of silage additive (P<0, 05) and their interactions (P<0, 05). Control silages had higher ammonium contents, except on the 182th day when silages with Lactobacillus plantarum had higher values. We conclude that application of additive reduced negative deamination activity that was undoubtedly present during whole storage period.

silage production ; Lactobacillus plantarum additive ; prolonged storage ; ammonium

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