engleski

The extremes of Rogoznica Lake (Eastern Adriatic coast): In-Depth Characterisation Of The Prokaryotic Community Beneath The Oxic Zone

A large fraction of all prokaryotes is thought to inhabit anaerobic environments, which represent a pool of genetically diverse microorganisms. Earth's oxygen-depleted zones are expanding areas that are the site of, and are implicating in, changing biogechemical cycles. Under these conditions, microbial populations are essential mediators of elemental cycling and energy transfer. Rogoznica Lake, situated on the eastern Adriatic coast, and known as Dragon’s Eye, is a highly eutrophic marine lake. With no fresh water inflow or drifts, it is considered a self-sustaining environment. This uniquely extreme ecosystem is euxinic (i.e. sulfidic) and meromictic. Due to its steep physico-chemical gradient (particularly oxygen and sulfide) it provides a suitable environment for the development of microaerophilic and anaerobic prokaryotic communities. Although extensively studied, nothing is yet known about the microbial community living beneath the oxic zone in Rogoznica Lake. We investigated the spatio-temporal dynamics of the active bacterial and archeal communities by sequencing of 16S rRNA amplicons generated from cDNA. Clear differences in the structure of the active community were observed across the vertical profile as well as during different seasons, all of which corresponded closely to environmental parameters. A complex and diverse distribution of microbial populations was revealed, supporting the idea that anaerobic degradation of organic matter in the Lake is driven by a range of taxa adjusted to environmental constraints.

Rogoznica Lake ; prokariotes ; hypoxia/anoxia ; biogeochemical cycles

nije evidentirano