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Environmental conditions shaping microbial mat community of the karst spring


Kolda, Anamarija; Petrić, Ines; Žutinić, Petar; Mejdandžić, Maja; Goreta, Gordana; Gottstein, Sanja; Ternjej, Ivančica; Gligora Udović, Marija
Environmental conditions shaping microbial mat community of the karst spring // Symposium on Aquatic Microbial Ecology Abstract Book / SAME15 / Gligora Udovič, Marija ; Orlić, Sandi ; Šolić, Mladen (ur.).
Zagreb: Ruđer Bošković Institute, 2017. str. 122-122 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Environmental conditions shaping microbial mat community of the karst spring

Autori
Kolda, Anamarija ; Petrić, Ines ; Žutinić, Petar ; Mejdandžić, Maja ; Goreta, Gordana ; Gottstein, Sanja ; Ternjej, Ivančica ; Gligora Udović, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Symposium on Aquatic Microbial Ecology Abstract Book / SAME15 / Gligora Udovič, Marija ; Orlić, Sandi ; Šolić, Mladen - Zagreb : Ruđer Bošković Institute, 2017, 122-122

ISBN
978-953-7941-18-5

Skup
Symposium on Aquatic Microbial Ecology (SAME15)

Mjesto i datum
Zagreb, Hrvatska, 03-08.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Microbial mat, Cyanobacteria, karst spring, NGS, ITS, 16S rRNA

Sažetak
Microbial mats are vertically stratified microbiomes consisting of benthic communities growing on solid substrates. They include communities of functionally distinct groups, largely consisting of prokaryotic Bacteria, Archaea and oxygenic filamentous phototrophic Cyanobacteria capable of thriving in extreme environmental conditions. Krčić is an intermittent karst spring situated in the Dinaric region of Croatia, characterized by high water- level fluctuations and long drought periods. Its physical and chemical properties support growth of specific microbial mat community capable of surviving such extreme hydrological events. We collected samples during different seasons and hydrological regimes in 2014 and 2015 in order to investigate the microbial mat diversity and understand the correlation of its genetic mark- up with environmental conditions. Genotypic diversity of Cyanobacteria was studied using specific primers amplifying ITS region, while Bacteria were studied using 16S rRNA as a phylogenetic marker. Analysis showed that Cyanobacteria was a predominant group in microbial mat (>50%), followed by Proteobacteria (α-Proteobacteria), Planctomycetes (Planctomycetaceae), Bacteroidetes (Cytophagaceae) and Verrucomicrobia (Verrucomicrobiaceae). During stable hydrological conditions a relatively constant microbial community was established, while summer conditions and long drought had a drastic effect on the community structure with enrichment of Firmicutes and Actinobacteria taxa and changes in abundances of dominant taxa. Detailed analysis revealed a switch in the cyanobacterial composition with Phormidium/Microcoleus genera being replaced with species closely related to Chroococcidiopsis, a desiccation- tolerant, endolithic cyanobacterium. Moreover, during stable community periods most of the cyanobacterial sequences related to uncultured Antarctic species, further implying that this ecosystem may comprise a unique assemblage of microbial species.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
351-01/13-01/108 MULTISEK
HRZZ-IP-2014-09-3494 - Mikrobna ekologija voda kao pokazatelj zdravstvenog stanja okoliša

Ustanove
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju

Kolda, Anamarija; Petrić, Ines; Žutinić, Petar; Mejdandžić, Maja; Goreta, Gordana; Gottstein, Sanja; Ternjej, Ivančica; Gligora Udović, Marija
Environmental conditions shaping microbial mat community of the karst spring // Symposium on Aquatic Microbial Ecology Abstract Book / SAME15 / Gligora Udovič, Marija ; Orlić, Sandi ; Šolić, Mladen (ur.).
Zagreb: Ruđer Bošković Institute, 2017. str. 122-122 (poster, međunarodna recenzija, sažetak, znanstveni)
Kolda, A., Petrić, I., Žutinić, P., Mejdandžić, M., Goreta, G., Gottstein, S., Ternjej, I. & Gligora Udović, M. (2017) Environmental conditions shaping microbial mat community of the karst spring. U: Gligora Udovič, M., Orlić, S. & Šolić, M. (ur.)Symposium on Aquatic Microbial Ecology Abstract Book / SAME15.
@article{article, year = {2017}, pages = {122-122}, keywords = {microbial mat, Cyanobacteria, karst spring, NGS, ITS, 16S rRNA}, isbn = {978-953-7941-18-5}, title = {Environmental conditions shaping microbial mat community of the karst spring}, keyword = {microbial mat, Cyanobacteria, karst spring, NGS, ITS, 16S rRNA}, publisher = {Ru\djer Bo\v{s}kovi\'{c} Institute}, publisherplace = {Zagreb, Hrvatska} }