The role of ABC-mediated transport in biocidal activity of natural and synthetic compounds on Anisakis pegreffii larvae (CROSBI ID 651474)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Trumbić, Željka ; Bušelić, Ivana ; Hrabar, Jerko ; Vrbatović, Anamarija ; Ujević, Ivana ; Roje Busatto, Romana ; Babić, Ivana ; Messina, Concetta Maria ; Mladineo, Ivona
engleski
The role of ABC-mediated transport in biocidal activity of natural and synthetic compounds on Anisakis pegreffii larvae
Anisakiasis, caused by accidental ingestion of live L3 stage larvae of Anisakis spp. through undercooked seafood, is a widespread, often underdiagnosed illness. One of the mechanisms commonly implicated in the development of drug resistance in nematodes is associated with the cellular efflux activity of ABC (ATP binding cassette) transmembrane transporters. Nematodes possess numerous homologues of ABCBs (Pgps and half-transporters) and ABCCs (MRPs) transporters with important physiological roles, aside from their role in multixenobiotic resistance. The scope of this study was to compare biocidal activity of two natural monoterpens (farnesol, nerolidol) and two synthetic widely-used anthelminthics (abamectin, levamisol) on Anisakis spp. larvae and the degree of involvement of ABC efflux mechanism in their pharmacokinetics. Ten Anisakis pegreffii larvae were exposed to growing concentrations of target chemicals ; experiment was conducted in six replicates with or without simultaneous administration of two inhibitors ; Valspodar (Pg-p inhibitor) and MK571 (MRP inhibitor). The concentration of target compound at which only 50% of larvae were alive (LC50) was determined: 135.9 μM for nerolidol, 134.4 μM farnesol, 0.3 μM abamectin and 0.84 for levamisol, significantly influenced by presence of one or both inhibitors for all chemicals except for abamectin. Toxicity curves were used to extrapolate doses where highest difference in larvicidal activity between the compound and one or both inhibitors was observed, suggesting it as point where ABC transporters elicit highest effect. Anisakis larvae were exposed to this concentration and sampled at 2, 6, 12 and 18 h after treatment for further analyses: quantification of Pg-p and MRP-like transcripts by real-time PCR, production of reactive oxygen species (ROS), intake of target compounds by HPLC, activity of transporters by Calcein-AM assay and effect on larval cytoskeleton by IHC and confocal microscopy. The results are discussed and provide integral view and understanding of the processes involved in larvicidal activity and protection mechanisms conferred by Anisakis pegreffii against tested compounds.
ABC transmembrane transporters, nerolidol, farnesol, abamectin, levamisol
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Podaci o prilogu
39-39.
2017.
objavljeno
Podaci o matičnoj publikaciji
Abstract Book of the 18th International Conference on Diseases of Fish and Shellfish
Mladineo, I.
Podaci o skupu
18th EAFP International Conference on Diseases of Fish and Shellfish
predavanje
04.09.2017-08.09.2017
Belfast, Ujedinjeno Kraljevstvo