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FT-IR spectroscopy as a method to detected cell surface components of Gluconobacter oxydans involved in the bacteria-OTA interaction


Jakopović, Željko; Mrvčić, Jasna; Pleadin, Jelka; Barišić, Lidija; Frece, Jadranka; Čanak, Iva; Markov, Ksenija
FT-IR spectroscopy as a method to detected cell surface components of Gluconobacter oxydans involved in the bacteria-OTA interaction // Journal of Biotechnology, volume 256, Supplement
Dubrovnik, Hrvatska, 2017. str. 66-66 (poster, međunarodna recenzija, sažetak, ostalo)


Naslov
FT-IR spectroscopy as a method to detected cell surface components of Gluconobacter oxydans involved in the bacteria-OTA interaction

Autori
Jakopović, Željko ; Mrvčić, Jasna ; Pleadin, Jelka ; Barišić, Lidija ; Frece, Jadranka ; Čanak, Iva ; Markov, Ksenija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Journal of Biotechnology, volume 256, Supplement / - , 2017, 66-66

Skup
EUROPEAN BIOTECHNOLOGY CONGRESS

Mjesto i datum
Dubrovnik, Hrvatska, 25-27.5.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Gluconobacter oxydans, OTA, cell wall components, FT-IR

Sažetak
Fourier Transform Infrared Spectroscopy (FT-IR) is an analytical technique, which provides unique spectrum for each bacterial species due to the stretching and bending vibrations of functional groups present in cell proteins, nucleic acids, lipids, sugars, and lipopolysaccharides. The potential of Gluconobacter oxydans to bind ochratoxin A (OTA) on surface and changes occurring on the cell surface due to mycotoxin exposure were investigated using the FT-IR. OTA was determined using liquid chromatography tandem mass spectrometry (LC-MS/MS) and the results suggest that G. oxydans is able to bind or degrade OTA by 81%. The results revealed capacity of OTA exposed cells to bind a high amount of the toxins is mirrored in the alteration of the original IR patterns, the latter alteration arising due the presence of numerous functional groups within the toxin structure. Major changes seen in the 1, 800-1, 600 cm-1, 1, 600-1, 450 cm-1 and 1, 300-1, 000 cm-1 regions, mainly correspond to carbonyl, aromatic and ether groups, respectively. These groups are abundant in OTA and increase the existing, or provide new spectrum bands. Additionally, very strong signals in the organic halogen compounds region at 870 cm–1, probably corresponds to the C-C1 vibration taking place in OTA.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija



POVEZANOST RADA


Ustanove
Hrvatski veterinarski institut, Zagreb,
Prehrambeno-biotehnološki fakultet, Zagreb