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One week of high salt dietary intake increased peripheral blood monocytes' intracellular hydrogen peroxide and peroxynitrite level in young healthy women

Aim: Previously we demonstrated that high salt dietary intake (HS diet) imapairs endothelial function in both micro- and macrocirculation in young healthy women, even in the absence of blood pressure changes. This study aimed to evaluate one week of HS diet effects on reactive oxygen species (ROS) production in human leukocytes. Meterials and Methods: Healthy women (N=15, age: 20±2) were taking HS diet (about 14g of NaCl/day) for 7 days. ROS production was measured in monocytes and lymphocytes from peripheral blood using flow cytometry (FACS Canto II, BD). Dichlorofluorescein diacetate (DCF-DA) was used to detect baseline and stimulated (phorbol 12- myristate 13-acetate (PMA)) intracellular hydrogen peroxide (H2O2) and peroxinitrite production, before and after HS diet. Data were expressed as the median fluorescence intensity (MFI). Results: 24h-urinary Na excretion and calculated salt intake confirmed that subjects conformed to the diet protocols (NaCl, g/day: pre HS 4.9±2.2 vs. post HS 12.2±5.8, P=0.003). HS diet significantly increased basal ROS production in monocytes compared to pre HS diet measurements (DCF-DA MFI pre HS 4.27±0.91 vs. post HS 4.96±1.3, P=0.024). There was no significant difference in ROS production in lymphocytes before and after HS diet (DCF-DA MFI pre HS 2.01±0.17 vs. post HS 1.95±0.12, P=0.184). PMA stimulation significantly increased ROS production in lymphocytes and monocytes before and after HS diet protocol. Conclusion: The results of present study demontrated that HS diet leads to leukocytes activation and increased oxidative stress which may contribute to endothelial activation and dysfunction even in the absence of blood pressure changes in young healthy persons. (HRZZ IP-2016-06-8744)

high salt diet, oxidative stress, leukocytes, flow citometry

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